• Home
  • Search Results
  • Norwegian Soils and Waters Contain Mesophilic, Plastic-Degrading Bacteria.

Norwegian Soils and Waters Contain Mesophilic, Plastic-Degrading Bacteria.

Microorganisms (2021-01-07)
Colin Charnock
ABSTRACT

Plastic pollution has become one of the most critical environmental issues, as rapidly increasing production, compounded by persistence of plastic wastes in the environment, are outpacing efforts to keep ecosystems plastic-free. A switch to plastics more amenable to microbial attack is one of several possible responses. Against this background, the current study describes the isolation, enumeration and polyphasic characterization of plastic-degrading bacteria present in Norwegian terrestrial and aquatic habits. It shows that these bacteria are present in relatively high numbers, and that plastic-degrading capabilities are found in several taxa, most especially Streptomyces. Some isolates wereable to degrade several plastics. Notably, a Rhodococcus sp. and a Streptomyces sp. degraded, respectively, four and six of the eight plastics investigated and a number of other polymers relevant for plastic blends. The paper also has a methodological aspect, presenting various approaches for assaying plastic-degrading properties and a PCR/sequencing-based approach for the identification of potential polyethylene terephthalate-degrading genes. A candidate gene was detected in several Streptomyces isolates. The study shows that Norwegian environments are a rich source of bacteria with the ability to degrade bioplastics possibly representing a natural remediation capacity, as well as a potential source of useful enzymes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
TWEEN® 20, viscous liquid
Sigma-Aldrich
Yeast Extract, for use in microbial growth medium
Sigma-Aldrich
Congo Red, Dye content ≥35 %
Sigma-Aldrich
Olive oil, highly refined, low acidity
Sigma-Aldrich
Poly[(R)-3-hydroxybutyric acid], natural origin
Sigma-Aldrich
3,6-Dimethyl-1,4-dioxane-2,5-dione, 99%
Sigma-Aldrich
Fluorescein (free acid), Dye content 95 %
Millipore
Trace Metal Mix A5 with Co, suitable for microbiology, sterile
Millipore
Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate, suitable for microbiology, NutriSelect® Basic
Sigma-Aldrich
Sodium carboxymethyl cellulose, average Mw ~700,000
Sigma-Aldrich
Starch, from potato, tested according to Ph. Eur.
Sigma-Aldrich
Glycolide, ≥99%
Sigma-Aldrich
N-Lauroylsarcosine sodium salt, BioXtra, ≥97% (TLC)
Millipore
Oatmeal agar, Microbiologically tested, NutriSelect® Basic
Sigma-Aldrich
Poly(ethylene succinate), average Mw 10,000
Corning® 15 mL centrifuge tubes, 15 mL centrifuge tubes, PET, conical bottom w/ plug seal cap, rack packed, sterile, natural, 50/rack, 500/cs