As your research uncovers the significant role of more and more biomarkers, you need a reliable partner for quality assays and consistent performance. Our MILLIPLEX® multiplex immunoassays are the largest portfolio of multiplex biomarker assays, based on Luminex® xMAP® multiplex assay technology, offering you consistent, high-quality results, so that you can do your best work while saving time, labor, and cost. Discover the benefits of these assays and see how they are being used to advance research for immunology, cancer, veterinary medicine, metabolism/endocrinology, and more.
A multiplex assay is an assay that combines tests for various analytes in a single panel. These assays have gained popularity for their advantages of saving sample volume and time by improving workflows when compared to assays that measure a single analyte, such as enzyme-linked immunosorbent assays (ELISAs).
MILLIPLEX® multiplex assays use the proprietary Luminex® xMAP® bead-based multiplex assay platform. This is how these multiplex assays work:
Figure 1 shows a general illustration of how these assays work.
Figure 1.MILLIPLEX® multiplex assay format based on Luminex® technology.
There are different detection systems for Luminex® multiplexing platforms, including the xMAP® INTELLIFLEX, Luminex® 200™, and FLEXMAP 3D® instruments. These Luminex® instruments use a flow cytometry-based system where beads flow in sheath fluid through a cell with a red laser (635 nm) identifying the bead region and a green laser (525 nm) quantifying the bound analyte “sandwich”. The instruments integrate key xMAP® detection components, such as lasers, optics, advanced fluidics, and high-speed digital signal processors.
Each individual microsphere is identified by its “bead signature,” (or bead region) and the result of its bioassay is quantified based on fluorescent reporter signals. We combine the power of Luminex® acquisition software with sophisticated analysis capabilities of Belysa® Immunoassay Curve Fitting software, integrating data acquisition and analysis seamlessly on all Luminex® instruments. Use of Belysa® Immunoassay Curve Fitting software affords the user advanced features not available within most data acquisition packages: auto-curve fitting functions, data hygiene rules, easy data visualization and standard curve comparison tools, with all data exportable in a variety of file formats.
The capability of adding multiplexed conjugated beads to each sample allows multiple assay results from each sample. Open architecture xMAP® technology enables the multiplexing of many types of bioassays, reducing time, labor, and costs over traditional methods.
Find the right platform for your research in our Luminex® multiplexing platform comparison article.
MILLIPLEX® multiplex assays contain all the components and reagents you need to simultaneously detect multiple analytes and offer the largest selection of kits, analytes, and species across many research areas. This includes:
The benefits of multiplex protein detection assays are endless, including saving on time, cost, reagents, and sample. With this broad portfolio, researchers can:
Discover more about the benefits of MILLIPLEX® multiplex immunoassays in this short video.
Most of all, the quality makes MILLIPLEX® assays stand out. From kit development and verification to manufacturing and quality control, researchers can rely on the quality we build into each kit to produce consistent, reliable results. In addition to the assay specifications listed in the protocol, we evaluate other performance criteria during our kit development and verification process: cross-reactivity, dilutional linearity, kit stability, and sample behavior (e.g., detectability and stability). We write the protocol with assay performance and your workflow in mind, making it easy to find the information you need. Learn more about the quality built into these kits in our Quality in MILLIPLEX® Multiplex Kits article.
We understand that some commercial multiplex kits may not provide everything needed for your research. To help address this, we also offer custom MILLIPLEX® multiplex assay services.
Our custom assay development services can help to:
MILLIPLEX® assays work seamlessly with Luminex® multiplex assay platforms, making them easy to run and analyze. We also offer ancillary equipment to enhance your multiplex workflows, such as the Biotek® Plate Washers.
Each MILLIPLEX® kit includes all necessary reagents to prepare the assay to read on your Luminex® instrument, just add your sample and water:
Note: MILLIPLEX® cell signaling kits have a different format to support the use of cell lysate samples.
Note: MILLIPLEX® antibody detection kits have a different format to measure antibodies in serum and plasma samples.
Multiplex immunoassays have a vast utility in a variety of research areas because they can provide a bigger picture of disease mechanisms and processes. MILLIPLEX® multiplex assays can be used in the following research areas:
Have questions about running MILLIPLEX® assays? Check out these articles for helpful tips:
Every year, thousands of researchers experience the benefits of MILLIPLEX® kits, publishing in scientific journals around the world. Check out the highlighted recent publications below separated by research area to see how researchers are using MILLIPLEX® assays.