Expertise in SMC® Technology
Digital Single Molecule Counting (SMC®) immunoassay technology provides unparalleled precision for the quantification of low and high abundance protein targets. Widely adopted by researchers and pharmaceutical organizations around the world, SMC® technology is being used to accelerate scientific discovery and therapeutic development programs across a variety of disease areas.
We understand that you don’t always have the time or resources to develop your own assay for your protein of interest or to test your critical samples, that’s where we can help.
Our Custom Assay / Sample Testing (CAST) services are powered by a scientific team with many years of combined immunoassay development experience, with their core expertise in SMC® technology. Whether you need fit-for-purpose sample testing, biomarker analysis using one of our currently available SMC® immunoassays, or development and manufacture of an immunoassay for your novel target of interest, our Custom Services team will partner with you to develop a project specific to your program requirements.
The team will work closely with you to understand and define the scope of the project and develop a customized project work plan. Throughout the project, you will receive both incremental progress updates and have easy direct access to the scientific team for questions and discussion related to the project. Final study reports are available at the end of the study and may be personalized to meet your specific needs. If your program requires GLP services, we can facilitate the technical method transfer to a CRO of your choice at any point in the Custom Assay Development process.
Our highly skilled team of scientists can accelerate your research by developing a customized SMC® assay for you. We adhere to the highest quality standards and provide solutions designed specifically for your research needs. We will work closely with you to develop a customized work plan to meet the scope of your project, ensuring complete confidentiality. Whether you require an assay for use in your own lab, for use with our sample testing services, or for transfer to a CRO of your choice, we have everything to meet your needs.
The purpose of our sample testing is to:
To accelerate your own assay development projects, we provide a variety of antibody derivatization services. Whether you provide your antibodies of choice or we source them for you, our team can derivatize and label microparticles with capture antibodies or fluorescently labeled detection antibodies. These reagents are optimized for the SMC® immunoassay system for use in either CAST development or for your development of your own assay utilizing our optimization kits in-house. Details are provided in Table 1.
Have more questions? Visit our CAST FAQs page to find answers to common questions.
Huntington’s Disease (HD) is a progressive brain disorder caused by a defect in the Huntingtin gene. HD is caused by a mutated form of the huntingtin gene, where excessive (more than 36) Glutamine repeats result in the formation of an unstable protein. This affects 5-10 in 100,000 people of European descent and is characterized by a decline in cognitive function as well as uncontrolled movement of the body. Mutant huntingtin protein (mHTT) is a key factor in neurodegeneration and accurate analysis in low levels is critical to accelerating research to ultimately find a cure for the disease.
Sample testing services through our SMC® CAST team were used to measure mHTT with a highly verified assay offering of:
Figure 1 depicts a typical standard curve using mHTT-Q45.
Figure 1.Typical standard curve using mHTT-Q45 [pg/mL].
Figure 2 shows how the mHTT assay differentiated between mutant and wild type protein through the use of SMC® technology.
Figure 2.Wild type HTT vs. mutant HTT using the SMC® mHTT assay. Mutant protein is shown in magenta and wild type protein is shown in blue.
Interested in our custom services? Consult with our custom assay team to learn more about how our extensive assay design experience can be customized for your project needs. Contact your local immunoassay sales specialists for more information.
For Research Use Only. Not For Use In Diagnostic Procedures.
See how researchers are using our custom services in the webinars below.