The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and then analyzed using HPLC or mass spectrophotometry.
The Global DNA Modification Kit (MDQ1), allows the researcher to monitor DNA methylation using a format similar to a sandwich ELISA assay. This is a format more friendly to biological researchers and is the first of its kind on the market.
Features and Benefits
Figure 1.Step-wise protocol for using MDQ1.
Figure 2.Comparison of two differently methylated lots of DNA with MDQ1. Methylation status of two lots of Sss I treated DNA was measured by LC-MS. Varying amounts of DNA was also analyzed via the MDQ1 kit. As demonstrated by the graph, the difference in methylation states can easily be detected and the signal generated is proportional to the amount of DNA used in the procedure.
Figure 3.Effect of Azacytadine on CHO Cell Global Methylation. Azacytadine, a known demethylating agent, was added directly to culture medium. Cells were harvested after 48 hours. DNA was isolated using GenElute™ and global methylation was measured. 40 ng of DNA from treated and non-treated CHO cells was analyzed.