HPLC Columns

Porous graphitic carbon (PGC) particles

Porous graphitic carbon (PGC) columns, like the Supel™ Carbon LC column, provide unique chromatographic properties that are unmatched by silica particle-packed columns.  The full pH range can be used as well as high temperature (> 200 °C), opening up new frontiers for chromatographers.

Superficially porous silica particles (SPP)/Fused Core^® Technology

Superficially porous silica columns deliver higher speed and efficiency than fully porous particles of the same size. Fused-Core^® HPLC columns like Ascentis^® Express and BIOshell™ can convert any HPLC system into a fast HPLC workhorse with maximum speed and performance.

Monolithic silica

• Monolithic, silica-based HPLC columns such as Chromolith^® HPLC columns allow rapid separations at very low column backpressure with high matrix tolerance and extended column lifetime, thus making them ideal products for high throughput and cost-efficient analyses of matrix rich samples.
• Chromolith^® HPLC columns are made from monolithic silica with a bimodal pore structure having 2 µm macropores and 13 nm mesopores. This structure leads to various key advantages to the column.
• Chromolith^® WP columns are wide pore (300 Å) monolithic silica columns made up of a single continuous rod of high purity, porous silica. These columns have shown great potential compared to conventional columns with consistent performance over a longer lifetime. In addition to various RP phases, a Protein A affinity column and an epoxy column, that enable customer specific modifications, are  also available.

Fully porous silica particles (FPP) Type B (high purity silica)

• Purospher^® STAR HPLC and UHPLC columns are best suited for the peak-tailing free separation of basic, neutral, and metal chelating samples. Their superior performance and stability allow maximum flexibility in method development with extended pH stability.
• Discovery^® HPLC columns are efficient for the analysis of biomolecules and small molecules. These columns have polar and non-polar bondings, with unique retention, selectivity, and no-bleed properties for LC-MS applications.
• The Ascentis^® bonded phases have a wide range of selectivity. Type B silica makes the column inert, stable, and reproducible. The high surface area of the silica is suitable for  preparative loading capacity.
• SeQuant^® HILIC HPLC columns (hydrophilic interaction liquid chromatography) carry densely bonded, zwitterionic functional groups. These HILIC columns are available in a variety of lengths, particle sizes, and pore dimensions while showing an exceptional reproducibility and robustness.
• Sepax^® HPLC columns are highly porous silica-based columns mainly utilized for peptides and protein analysis.

Fully porous silica particles (FPP) Type A (conventional silica)

• Supelcosil^® silica-based HPLC column line has a wide range of bonded phase chemistries, with the particle sizes and column configurations ranging from microbore to preparative scale. These columns show great efficiency for polar, nonpolar, and charged analytes.
• LiChrospher^® and Superspher^® HPLC columns are highly versatile and reliable, proven in thousands of established HPLC methods.
• LiChrosorb^® HPLC columns are packed with irregular silica particles ranging from analytical scale 5 µm to large particles sized for production scale.
• TSKgel^® HPLC and UHPLC columns are available for gel filtration, gel permeation, ion exchange, reversed-phase, hydrophobic interaction, hydrophilic interaction, and affinity chromatography. (only available in the US and Europe)

Fully porous polymeric particles

• apHera™ reversed-phase and amino HPLC columns are stable at the pH range 2-12 and are amenable to washing with alkaline solutions. They are ideal for the analysis of peptides, small proteins, carbohydrates, polar drugs, and organic acids.
• Supelcogel^® HPLC columns are packed with polystyrene-divinylbenzene co-polymer (PS-DVB) making them chemically stable. The two variations of PS-DVB co-polymer (6% and 8% cross-linked) maximize the separation process of the samples.
• Hamilton Polymeric Reversed Phase HPLC columns are extremely inert, efficient, and durable, with high mechanical and thermal stability.
• TSKgel^® HPLC and UHPLC columns are available for the separation of proteins, peptides, and other biomolecules. The columns containing silica and methacrylate particles with different pore sizes can accommodate analytes with a wide range of molecular weights. (only available in the US and Europe)

Chiral HPLC Columns

We have a wide range of chiral HPLC columns for various applications.

• Astec CYCLOBOND™ Chiral columns have bonded cyclodextrin phases that allow selective chemical separation of organic molecules.
• Astec CHIROBIOTIC^TM Chiral columns are based on macrocyclic glycoproteins bonded to high purity silica gels available for LC-MS chiral applications.
• Astec Cellulose DMP Chiral columns with spherical, high-purity porous silica coated with DMPC (3,5-dimethylphenyl carbamate); can separate a wide range of chiral compounds under normal phase, polar organic, and SFC conditions.
• Astec Copper Ligand Exchange Chiral columns are beneficial for resolving hydroxy acids, amino acids, bi-functional racemates, and other amines.

Tutorials

Learn how to connect HPLC columns and cartridges with the appropriate pre-columns, holders, and guard cartridges.

*Tosoh Bioscience columns are available in the following countries: