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L0543
LB Agar Kanamycin-50, Plates
pre-poured LB agar plates with kanamycin sulfate
Synonym(s):
kanamycin agar plates
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| Size/SKU | Availability | Price |
|---|---|---|
10 ea | Available to ship TODAYfromMILWAUKEE | $54.20 |
About This Item
UNSPSC Code:
41121800
Sterility:
sterile; autoclaved
Suitability:
selective for Escherichia coli, selective for coliforms
$54.20
Available to ship TODAYDetails
grade
Molecular Biology
Quality Segment
sterility
sterile; autoclaved
form
plates (10 cm), plates (10cm, 20-25ml each)
capacity
20-25 mL
storage temp.
2-8°C
suitability
selective for Escherichia coli, selective for coliforms
General description
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Application
Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens and E.coli JM83 strain with pGD103 plasmid.
Features and Benefits
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
Other Notes
15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
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This Item | |||
|---|---|---|---|
| description pre-poured LB agar plates with kanamycin sulfate | description Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture. | description Liquid microbial growth medium | description Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture |
| sterility sterile; autoclaved | sterility non-sterile | sterility sterile; 0.2 μm filtered | sterility non-sterile |
| suitability selective for Escherichia coli, selective for coliforms | suitability nonselective for coliforms, nonselective for Escherichia coli | suitability nonselective for coliforms, nonselective for Escherichia coli | suitability nonselective for coliforms, nonselective for Escherichia coli |
| storage temp. 2-8°C | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| capacity 20-25 mL | capacity - | capacity - | capacity - |
| Quality Level 200 | Quality Level 200 | Quality Level 400 | Quality Level 200 |
Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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What is the Department of Transportation shipping information for this product?
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Why do the LB-agar plates with antibiotics have such a short expiration date?
1 answer-
The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
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