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C9658

Carboxypeptidase G from Pseudomonas sp.

lyophilized powder, ≥3 units/mg protein

Synonym(s):

γ-Glutamyl hydrolase

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5 units

Available to ship TODAYfromMILWAUKEE

$270.00
20 units

Available to ship TODAYfromMILWAUKEE

$804.00
$683.40

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-978-1
MDL number:
EC Number:
Specific activity:
≥3 units/mg protein

$270.00


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form

lyophilized powder

Quality Segment

specific activity

≥3 units/mg protein

mol wt

homodimer ~90 kDa

composition

Protein, ~70% biuret

solubility

H2O: soluble 0.5 mg/mL

storage temp.

−20°C

Application

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Biochem/physiol Actions

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.

Physical form

Contains sodium acetate salt.

Preparation Note

Chromatographically purified
Solutions should be prepared fresh prior to use.

Other Notes

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

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C9584C9268C3888
description

lyophilized powder, ≥3 units/mg protein

description

lyophilized powder

description

(Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution

description

lyophilized powder, ≥50 units/mg protein

specific activity

≥3 units/mg protein

specific activity

≥125 units/mg protein

specific activity

≥50 units/mg protein

specific activity

≥50 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

ready-to-use solution

form

lyophilized powder

solubility

H2O: soluble 0.5 mg/mL

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

homodimer ~90 kDa

mol wt

34,000 Da± 600

mol wt

~35 kDa

mol wt

61 kDa

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

200


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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