If you have a 3X FLAG-tagged protein, then you will need to use the 3X FLAG peptide. If you have a 1X FLAG-tagged protein, you can use the 1X FLAG peptide or the 3X FLAG peptide. We have not noticed a significant difference in elution efficiency by using a 3X FLAG peptide on a 1X FLAG-tagged protein.
F2426
EZview™ Red 抗FLAG®M2抗体 アフィニティーゲル
clone M2
別名:
モノクローナル抗FLAG® M2抗体 マウス宿主抗体, 抗ddddk, 抗dykddddk
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
1 mL | 在庫from市川市大町368 | ￥107,000 |
この商品について
NACRES:
NA.32
UNSPSC Code:
12352203
Clone:
M2, monoclonal
Technique(s):
affinity chromatography: suitable, immunoprecipitation (IP): suitable
Application:
IP, affinity chromatography
Citations:
204
Quality Segment
clone
M2, monoclonal
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
matrix
4% agarose bead; 45-165μm bead size
isotype
IgG1
capacity
≥0.6 mg/mL, gel binding capacity
shipped in
wet ice
storage temp.
−20°C
General description
EZ view Red Anti-FLAG M2アフィニティーゲルは、4%アガロースビーズに共有結合した抗FLAG M2抗体から構成される樹脂です。アフィニティーゲルは、免疫沈降アッセイの調製において、FLAG-タグ化タンパク質の精製を目的として、細胞溶解物や組織などの試料にFLAG融合タンパク質を結合させるために使用されます。赤色の色素により視認性が高まり、より効率的に結果を得ることができます。アガロースビーズは、N-末端、Met-N-末端およびC-末端で、FLAG融合タンパク質、3xFLAG-タグ化融合タンパク質に結合します。
Application
FLAG-および3xFLAG-タグ付き融合タンパク質の免疫沈降（IP）。
溶出 - FLAGペプチド、グリシン、pH 3.5、3x FLAGペプチド
私たちのFLAG®アプリケーションポータルで製品の詳細をご覧ください。
溶出 - FLAGペプチド、グリシン、pH 3.5、3x FLAGペプチド
私たちのFLAG®アプリケーションポータルで製品の詳細をご覧ください。
Biochem/physiol Actions
N末端,、Met-N末端、C末端にタグが位置するFLAG融合タンパク質および3×FLAG融合タンパク質の精製に適しています。
Physical form
50%グリセロ-ル, 15ppm Kathonを含むPBSで調製した1:1 (v/v)懸濁液
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
FLAG® アフィニティゲル、FLAG® タグ、3 x FLAG® タグ、DYKDDDDK タグ
1 of 1
当該品目 | |||
|---|---|---|---|
| clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal | clone - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable |
| isotype IgG1 | isotype IgG1 | isotype IgG1 | isotype - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
保管分類
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, should I use a 3X FLAG peptide or a 1X FLAG peptide to elute my protein?
1 回答-
役に立ちましたか？
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When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, I see bands at 20-25 kDa and 50-60 kDa appearing in my Westerns that are not my FLAG-tagged protein. How can I prevent this?
1 回答-
As a result of the conjugation, there may be some M2 antibody that is not conjugated to the resin, but is associated with the resin and may appear in acid elutions as heavy and light chain when using the anti-mouse IgG conjugated secondary antibody. We recommend a acid wash (0.1 M glycine-HCL pH 3.5) and neutralization of the resin (do not allow the acid wash to sit on the resin longer than 20 minutes) prior to applying the lysate. Another way to avoid this is to use a directly conjugated FLAG antibody for detection such as product A8592 ant-FLAG M2 HRP, or the rabbit anti-FLAG polyclonal antibody, F7425.
役に立ちましたか？
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When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, I have a lot of non-specific proteins that are eluting with my FLAG-tagged protein. How can I get rid of these?
1 回答-
One way to remove non-specific proteins is to pre-bind the protein lysate with unconjugated resin. We recommend product 4B200 for this purpose. Other methods would be to increase the stringency of the washes by increasing salt concentration (the resin can tolerate up to 1M NaCl) or including detergents that are compatible with the resin.
役に立ちましたか？
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What is the binding capacity of the Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, resin?
1 回答-
The binding capacity of the resin must be ? 0.6 mg/mL to meet specifications. This capacity will vary from lot to lot.
役に立ちましたか？
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When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, how can I elute my protein?
1 回答-
Elution with the peptide is the most gentle method. Acid elution (0.1 M glycine-HCL pH 3.5) is a more stringent method of elution, and should be evaluated for its effect on your protein if it is to be used in downstream applications. Boiling the resin in sample buffer is the most denaturing condition. If this condition is used, the resin cannot be re-used, due to the presence of SDS and/or reducing agents.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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