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MilliporeSigma

A4596

Millipore

ANTI-FLAG® M1 Agarose Affinity Gel

Synonym(s):

Anti-ddddk, Anti-dykddddk

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1 ML
$685.00
5 ML
$1,550.00
10 ML
$2,810.00
25 ML
$5,590.00

$685.00


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1 ML
$685.00
5 ML
$1,550.00
10 ML
$2,810.00
25 ML
$5,590.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.32

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$685.00


Check Cart for Availability

Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
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conjugate

agarose conjugate

antibody product type

primary antibodies

form

suspension

isotype

IgG12b

capacity

≥0.6 mg/mL, gel binding capacity

storage temp.

−20°C

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This Item
A2220F2426F3040
conjugate

agarose conjugate

conjugate

agarose conjugate

conjugate

-

conjugate

unconjugated

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

suspension

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous solution

isotype

IgG12b

isotype

IgG1

isotype

IgG1

isotype

-

antibody product type

primary antibodies

antibody product type

primary antibodies

antibody product type

-

antibody product type

primary antibodies

capacity

≥0.6 mg/mL, gel binding capacity

capacity

>0.6 mg/mL, resin binding capacity (FLAG-BAP)

capacity

≥0.6 mg/mL, gel binding capacity

capacity

-

General description

ANTI-FLAG® M1 Agarose Affinity Gel is a purified IgG2B monoclonal antibody covalently attached to agarose.

Specificity

Binding specificity: Free N-Terminus of FLAG sequence
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Application

For purification of N-terminal FLAG fusion proteins. Since binding is Ca2+-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.

immunoprecipitation (IP): suitable

Elution - FLAG peptide, Glycine, pH 3.5 EDTA

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step.
  • Fusion protein may be eluted from affinity resin by mild elution with EDTA.
  • A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins.

Physical form

Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide

Other Notes

ANTI-FLAG® M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

1–6 of 6 Questions  
  1. Bonjour, pouvez vous m'expliquer pour quoi la résine anti Flag M1 est calcium dépendante contrairement à la résine antiFlag M2 sachant que les séquences de reconnaissances sont les mêmes?

    1 answer
    1. Anti-Flag M1 antibody is specific for a calcium complex of the FLAG peptide. M1 and M2 are different clones. The ANTI-FLAG M1 monoclonal antibody binds to proteins with a FLAG marker at the free N-terminus. The ANTI-FLAG M2 monoclonal antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

      Helpful?

  2. Can I use Phosphate Buffered Saline (PBS) instead of Tris Buffered Saline (TBS) with Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel?

    1 answer
    1. We do not recommend the use of phosphate buffers since calcium is required for binding.  This can result in the formation of insoluble calcium phosphates in the presence of PBS.

      Helpful?

  3. How much calcium is required for binding of my FLAG-tagged protein to Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel?

    1 answer
    1. 10 mM calcium chloride is recommended for binding.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What reagents are compatible with Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel?

    1 answer
    1. The ANTI-FLAG M1 Affinity Gel is resistant to the following detergents: 5.0 % Tween-20; 5.0 % Triton X-100; 0.1 % NP-40; 0.1 % CHAPS and 0.2 % digitonin. It can also be used with 1.0 M NaCl or 1.0 M urea. Do not use the gel in the presence of SDS, deoxycholate or guanidine HCl.

      Helpful?

  6. Will Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel, work for my C-terminal FLAG-tagged protein?

    1 answer
    1. No.  The M1 FLAG antibody resin will only bind proteins that are free N-terminal FLAG tagged proteins.  It will not recognize C-terminal FLAG tagged proteins or Met-N-terminal FLAG tagged proteins.

      Helpful?

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