Anti-Flag M1 antibody is specific for a calcium complex of the FLAG peptide. M1 and M2 are different clones. The ANTI-FLAG M1 monoclonal antibody binds to proteins with a FLAG marker at the free N-terminus. The ANTI-FLAG M2 monoclonal antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
A4596
ANTI-FLAG® M1 Agarose Affinity Gel
Synonym(s):
Anti-ddddk, Anti-dykddddk
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1 ML
$733.00
5 ML
$1,660.00
10 ML
$3,000.00
25 ML
$5,990.00
About This Item
NACRES:
NA.32
UNSPSC Code:
12352203
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conjugate
agarose conjugate
antibody product type
primary antibodies
form
suspension
isotype
IgG12b
capacity
≥0.6 mg/mL, gel binding capacity
storage temp.
−20°C
Quality Level
1 of 4
This Item | A2220 | F2426 | F3040 |
|---|---|---|---|
| conjugate agarose conjugate | conjugate agarose conjugate | conjugate - | conjugate unconjugated |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| form suspension | form buffered aqueous glycerol solution | form - | form buffered aqueous solution |
| isotype IgG12b | isotype IgG1 | isotype IgG1 | isotype - |
| antibody product type primary antibodies | antibody product type primary antibodies | antibody product type - | antibody product type primary antibodies |
| capacity ≥0.6 mg/mL, gel binding capacity | capacity >0.6 mg/mL, resin binding capacity (FLAG-BAP) | capacity ≥0.6 mg/mL, gel binding capacity | capacity - |
Biochem/physiol Actions
Binding specificity: Free N-Terminus of FLAG sequence
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Features and Benefits
- Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step.
- Fusion protein may be eluted from affinity resin by mild elution with EDTA.
- A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins.
General description
ANTI-FLAG® M1 Agarose Affinity Gel is a purified IgG2B monoclonal antibody covalently attached to agarose.
Other Notes
ANTI-FLAG® M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.
Physical form
Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Application
For purification of N-terminal FLAG fusion proteins. Since binding is Ca2+-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.
Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.
immunoprecipitation (IP): suitable
Elution - FLAG peptide, Glycine, pH 3.5 EDTA
Learn more product details in our FLAG® application portal.
Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.
immunoprecipitation (IP): suitable
Elution - FLAG peptide, Glycine, pH 3.5 EDTA
Learn more product details in our FLAG® application portal.
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Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Bonjour, pouvez vous m'expliquer pour quoi la résine anti Flag M1 est calcium dépendante contrairement à la résine antiFlag M2 sachant que les séquences de reconnaissances sont les mêmes?
1 answer-
Helpful?
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What reagents are compatible with Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel?
1 answer-
The ANTI-FLAG M1 Affinity Gel is resistant to the following detergents: 5.0 % Tween-20; 5.0 % Triton X-100; 0.1 % NP-40; 0.1 % CHAPS and 0.2 % digitonin. It can also be used with 1.0 M NaCl or 1.0 M urea. Do not use the gel in the presence of SDS, deoxycholate or guanidine HCl.
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How much calcium is required for binding of my FLAG-tagged protein to Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel?
1 answer-
10 mM calcium chloride is recommended for binding.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can I use Phosphate Buffered Saline (PBS) instead of Tris Buffered Saline (TBS) with Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel?
1 answer-
We do not recommend the use of phosphate buffers since calcium is required for binding. This can result in the formation of insoluble calcium phosphates in the presence of PBS.
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Will Product A4596, ANTI-FLAG® M1 Agarose Affinity Gel, work for my C-terminal FLAG-tagged protein?
1 answer-
No. The M1 FLAG antibody resin will only bind proteins that are free N-terminal FLAG tagged proteins. It will not recognize C-terminal FLAG tagged proteins or Met-N-terminal FLAG tagged proteins.
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