A4596

ANTI-FLAG^® M1 Agarose Affinity Gel

• Synonym(s): Anti-ddddk, Anti-dykddddk
• NACRES: NA.32

Properties

• conjugate: agarose conjugate
• Quality Level: 200
• antibody product type: primary antibodies
• form: suspension
• isotype: IgG12b
• capacity: ≥0.6 mg/mL, gel binding capacity
• storage temp.: −20°C

Description

General description

ANTI-FLAG^® M1 Agarose Affinity Gel is a purified mouse IgG2B monoclonal antibody covalently attached to agarose.

Specificity

Binding specificity: Free N-Terminus of FLAG sequence
N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Application

For purification of N-terminal FLAG fusion proteins. Since binding is Ca²⁺-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.

immunoprecipitation (IP): suitable

Elution - FLAG peptide, Glycine, pH 3.5 EDTA

Browse additional application references in our FLAG^® Literature portal.

Features and Benefits

• Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step.
• Fusion protein may be eluted from affinity resin by mild elution with EDTA.
• A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins.

Physical form

Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide

Other Notes

ANTI-FLAG^® M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable