FLAGIPT1
FLAG® Immunoprecipitation Kit
Synonym(s):
Anti-ddddk, Anti-dykddddk
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1 KIT
$1,790.00
$1,790.00
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
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usage
kit sufficient for 50 reactions
analyte chemical class(es)
proteins (FLAG® Affinity Gels)
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
storage temp.
−20°C
Quality Level
1 of 4
This Item | F2426 | A2220 | F3290 |
|---|---|---|---|
| technique(s) immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) - |
| usage kit sufficient for 50 reactions | usage - | usage - | usage - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| analyte chemical class(es) proteins (FLAG® Affinity Gels) | analyte chemical class(es) proteins | analyte chemical class(es) proteins | analyte chemical class(es) - |
Application
Learn more product details in our FLAG® application portal.
Features and Benefits
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3xFLAG™ peptide provides efficient and gentle elution through direct competition
General description
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Other Notes
All prodedures to be performed at 2-8°C.
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2
Storage Class
10 - Combustible liquids
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