Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

I9888

Monoclonal Anti-Human IgG4 (pFc′ specific) antibody produced in mouse

clone HP-6023, ascites fluid

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
0.2 mL

Available to ship TODAYfromMILWAUKEE

$234.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00164659
Conjugate:
unconjugated
Clone:
HP-6023, monoclonal
Application:
ELISA (i)
Citations:
19

$234.00

Available to ship TODAYDetails

​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6023, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:2,000

isotype

IgG3

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. Human IgG4 is rarely found in the serum. It has the ability to bind FcγRI with high affinity. High levels of IgG4 in the serum can be pathogenic and may lead to Wegener′s granulomatosis.
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Monoclonal Anti-Human IgG4 (pFc′ specific) antibody is specific for an epitope on the pFc′ region of IgG4. The product does not react with IgG1, IgG2 and IgG3. This monoclonal antibody has been cited as the most widely applicable IgG4 specific reagent by the IUIS/WHO study.
IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

Monoclonal Anti-Human IgG4 (pFc′ specific) antibody has been used in immunoblotting.
Monoclonal Anti-Human IgG4 (pFc′ specific) antibody can be used for immunoblotting and Indirect Immunoperoxidase Assay.
This clone has been singled out as most widely applicable by the IUIS/WHO study.

Biochem/physiol Actions

IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differs from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
I9388I7385A8667
Monoclonal Anti-Human IgG4 (pFc′ specific) antibody produced in mouse clone HP-6023, ascites fluid

Sigma-Aldrich

I9888

Monoclonal Anti-Human IgG4 (pFc′ specific) antibody produced in mouse

Monoclonal Anti-Human IgG1 (Fc specific) antibody produced in mouse clone HP-6001, ascites fluid

Sigma-Aldrich

I9388

Monoclonal Anti-Human IgG1 (Fc specific) antibody produced in mouse

Anti-Human IgG4 antibody, Mouse monoclonal clone HP-6025, purified from hybridoma cell culture

Sigma-Aldrich

I7385

Anti-Human IgG4 antibody, Mouse monoclonal

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A8667

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat

description

clone HP-6023, ascites fluid

description

clone HP-6001, ascites fluid

description

clone HP-6025, purified from hybridoma cell culture

description

IgG fraction of antiserum, buffered aqueous solution

biological source

mouse

biological source

mouse

biological source

mouse

biological source

goat

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

IgG fraction of antiserum

technique(s)

indirect ELISA: 1:2,000

technique(s)

indirect ELISA: 1:3,000

technique(s)

indirect ELISA: 1:5,000, immunofluorescence: suitable

technique(s)

dot blot: 1:80,000, direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

peroxidase conjugate

isotype

IgG3

isotype

IgG2b

isotype

IgG1

isotype

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters