White precipitation occurs due to the formation of paraformaldehyde, a common phenomenon in formaldehyde solutions. This product contains 10-15% methanol as a stabilizer to slow down the process. Ideally, storage should be at 25°C, as lower temperatures accelerate polymerization. The formation of paraformaldehyde is reversible, so the product may still be usable. To achieve a clear solution, gently heat the cloudy solution up to 30°C for 1 to 2 hours, taking care not to overheat, as this can cause degradation.
252549
Formaldeído
Formaldehyde solution
ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Sinônimo(s):
Formalina
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About This Item
Fórmula linear:
HCHO
Número CAS:
Peso molecular:
30.03
Beilstein:
1209228
Número MDL:
Código UNSPSC:
12352114
ID de substância PubChem:
NACRES:
NA.21
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
grau
ACS reagent
Nível de qualidade
densidade de vapor
1.03 (vs air)
pressão de vapor
52 mmHg ( 37 °C)
52 mmHg ( 37 °C)
Formulário
liquid
temperatura de autoignição
572 °F
contém
10-15% Methanol as stabilizer (to prevent polymerization)
concentração
36.5-38.0%
37 wt. % in H2O
Impurezas
≤0.006 meq/g Titr. acid
resíduo de ignição
≤0.005%
cor
APHA: ≤10
índice de refração
n20/D 1.3765
solubilidade
water: soluble
densidade
1.09 g/mL at 25 °C (lit.)
traços de ânion
chloride (Cl-): ≤5 ppm
sulfate (SO42-): ≤0.002%
traços de cátion
Fe: ≤5 ppm
heavy metals: ≤5 ppm (by ICP-OES)
adequação
suitable for manufacturing use (Laboratory chemicals)
cadeia de caracteres SMILES
[H]C([H])=O
InChI
1S/CH2O/c1-2/h1H2
chave InChI
WSFSSNUMVMOOMR-UHFFFAOYSA-N
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Categorias relacionadas
Descrição geral
Aplicação
Formaldehyde solution has been used as a fixing agent to fix cells during immunofluorescence imaging and for cross-linking cells during chromatin immunoprecipitation (ChIP) assay.[3]
forma física
This product is a solution of approximately 37% by weight of formaldehyde gas in water.
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Nº do produto
Descrição
Preços
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
Órgãos-alvo
Eyes,Central nervous system, Respiratory system
Código de classe de armazenamento
3 - Flammable liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
132.8 °F - closed cup
Ponto de fulgor (°C)
56 °C - closed cup
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Hi! My formaldehyde solution presents a white precipitate, and I think it can be paraformaldehyde. Can it still be used as formaldehyde, or is there a way to redisolve it and make it functional again? Thanks!
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Can you store formaldehyde in plastic?
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This product is packaged in glass bottles. Generally, formaldehyde solution can be used in plastic containers. However, it is advised that the unused material be preserved in its original packaging.
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What type of packaging for different packages?
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This product is packaged in amber glass bottles with polypropylene caps with HDPE liners. The bottle sizes are as follows:
252549-25 ML in 50 ml bottles
252549-100 ML in 100 ml bottles
252549-500ML in 500 ml bottles
252549-1L in 1L bottles
252549- 4L in 4L bottlesHelpful?
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Can formaldehyde solution 37% be diluted to 10% formalin for fixing cells for staining? Do you dilute it with around 2.7 part water? Thank you!
1 answer-
How much water should be added to 100mL of a 37% solution to reduce its strength to a 10% solution?
Use C1 x V1= C2 x V2
37% x 100ml = 10% x V2
Therefore V2 = (37% x 100ml) / 10%
= 370 mLSubtract the total original volume from the new volume.
370 mL - 100 mL = 270 mL
So to reduce 100mL of 37% to 10% solution, add 270 mL of water to 100 mL of Formaldehyde solution - 252549-25ML.Helpful?
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Which formaldehyde solution is recommended for use with the HT102A Reticulum kit?
1 answer-
When a silver stain procedure recommends a 10% formalin solution, it is referring to a 10% aqueous formalin solution, which is prepared by diluting 1 part of 37% to 40% formaldehyde with 9 parts deionized water. For histology, it is advisable to use ACS reagents when the product meets ACS Specifications. Suitable products for this purpose would be product numbers 252549 or FX0410. If a 37% to 40% formaldehyde solution is available, it should work; however, it's important to avoid using old bottles of this solution that have been in the lab for several years, as impurities can form over time. Solutions used in histological procedures should typically be replaced every 2 years to ensure effectiveness.
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Is the methanol content in the CoA expressed as volume percent or weight percent?
1 answer-
The methanol content is expressed as weight percent.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do you offer any aqueous paraformaldehyde solutions?
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Product No. 252549 is an aqueous solution of formaldehyde with methanol present to prevent polymerization of the formaldehyde to paraformaldehyde. Over time (e.g., if a lot of the methanol evaporates), the solution may precipitate the insoluble paraformaldehyde. To break up that polymer, gentle heating should be sufficient. The polymer form has very low solubility in water.
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