Merck
All Photos(2)

71736

Sigma-Aldrich

Sodium dodecyl sulfate solution

BioUltra, for molecular biology, 10% in H2O

Synonym(s):
Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate solution
Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
Molecular Weight:
288.38
Beilstein:
3599286
Número MDL:
eCl@ss:
39093306
ID de substância PubChem:
NACRES:
NA.26

grau

for molecular biology

Nível de qualidade

200

descrição

anionic

linha de produto

BioUltra

forma

liquid

peso molecular

288.38 g/mol

concentração

10% in H2O

technique(s)

HPLC: suitable
protein quantification: suitable

Impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.0-6.5

densidade

1.01 g/mL at 20 °C

traços de ânion

chloride (Cl-): ≤50 mg/kg
phosphate (PO43-): ≤1 mg/kg

traços de cátion

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

Absorção UV

λ: 260 nm Amax: 0.3
λ: 280 nm Amax: 0.2

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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Aplicação

Sodium dodecyl sulfate solution has been used:
  • in the preparation of 2x extraction buffer[1]
  • as a component in elution buffer for immunoprecipitation[2]
  • in SDS–polyacrylamide gel electrophoresis (PAGE) analysis[3][4]

Ações bioquímicas/fisiológicas

Sodium dodecyl sulfate (SDS)/lauryl sulfate, is an ionic detergent has the ability to disrupt biological membranes. It can block ribonuclease (RNase) and deoxyribonuclease (DNase) activity. SDS is one of the main constituents of several reagents, that are used to purify nucleic acids.[5] It is also considered as a permeability enhancer in oral formulations.[6]

Outras notas

In large-scale phenol extraction of RNA - promotes the dissociation of protein from nucleic acids when extracting from biological material[7]

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Skin Irrit. 2

Código de classe de armazenamento

12 - Non Combustible Liquids

WGK

WGK 2

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificate of Analysis

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Certificate of Origin

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