L3771

Sigma-Aldrich

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

Sinônimo(s):
Lauryl sulfate sodium salt, Sodium lauryl sulfate, Dodecyl sulfate sodium salt, SDS, Dodecyl sodium sulfate
Fórmula linear:
CH3(CH2)11OSO3Na
Número CAS:
Peso molecular:
288.38
Beilstein/REAXYS Number:
3599286
Número EC:
Número MDL:
eCl@ss:
39093306
ID de substância PubChem:
NACRES:
NB.22

Nível de qualidade

200

grau

for molecular biology

descrição

anionic

linha de produto

BioReagent

teor

≥98.5% (GC)

peso molecular

micellar 18,000

número de agregação

62

aplicação(ões)

electrophoresis: suitable

CMC

7-10 mM (20-25°C)

pf

204-207 °C (lit.)

temperatura de transição

cloud point >100 °C

solubilidade

water: soluble

traços de ânion

chloride (Cl-): ≤500 ppm
phosphate (PO43-): ≤10 ppm

traços de cátion

heavy metals (as Pb): ≤10 ppm

absorção

≤0.1 at 260 at 3%
≤0.1 at 280 at 3%

HLB

40

adequação

suitable for electrophoresis

atividade externa

DNase, RNase, none detected

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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Descrição geral

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.

Aplicação

Sodium dodecyl sulfate has been used:
  • in chromatin immunoprecipitation
  • in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
  • as a component of radioimmunoprecipitation assay buffer for immunoblotting
Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.
Anionic detergent

Embalagem

25, 100, 500 g in poly bottle
1 kg in poly bottle

Nota de análise

Tested for use in denatured polyacrylamide gel electrophoresis.

Palavra indicadora

Danger

hazcat

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

storage_class_code

4.1B - Flammable solid hazardous materials

WGK Alemanha

WGK 2

Ponto de fulgor (ºF)

338.0 °F

Ponto de fulgor (ºC)

170 °C

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

Certificado de análise

Certificado de origem

Shane Stegeman et al.
Endocrine-related cancer, 22(2), 265-276 (2015-02-12)
The oncogene MDM4, also known as MDMX or HDMX, contributes to cancer susceptibility and progression through its capacity to negatively regulate a range of genes with tumour-suppressive functions. As part of a recent genome-wide association study it was determined that...
Kazuya Ishikawa et al.
Nature communications, 5, 5430-5430 (2014-11-13)
Pathogen effector proteins are delivered to host cells to suppress plant immunity. However, the mechanisms by which effector proteins function are largely unknown. Here we show that expression of XopP(Xoo), an effector of rice pathogen Xanthomonas oryzae pv. oryzae, in...
S Bavamian et al.
Molecular psychiatry, 20(5), 573-584 (2015-01-28)
Bipolar disorder (BD) is a heritable neuropsychiatric disorder with largely unknown pathogenesis. Given their prominent role in brain function and disease, we hypothesized that microRNAs (miRNAs) might be of importance for BD. Here we show that levels of miR-34a, which...
Takahiro Kishikawa et al.
Oncotarget, 6(10), 8339-8352 (2015-04-01)
Reduced expression of microRNA122 (miR122), a liver-specific microRNA, is frequent in hepatocellular carcinoma (HCC). However, its biological significances remain poorly understood. Because deregulated amino acid levels in cancers can affect their biological behavior, we determined the amino acid levels in...
Chen Li Chew et al.
Cancer discovery, 5(7), 740-751 (2015-04-18)
The phosphatases PTEN and INPP4B have been proposed to act as tumor suppressors by antagonizing PI3K-AKT signaling and are frequently dysregulated in human cancer. Although PTEN has been extensively studied, little is known about the underlying mechanisms by which INPP4B...
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