Direct evidence of spatially selective iron mineralization using an immobilized ferritin protein cage.

Journal of nanoscience and nanotechnology (2014-04-17)
Koichiro Uto, Kazuya Yamamoto, Naoko Kishimoto, Masahiro Muraoka, Takao Aoyagi, Ichiro Yamashita
RESUMO

(Apo)ferritins are cage-shaped proteins which have recently received a great deal of attention because the inner cavity of the protein shell can be used as a size-restricted reaction field for the synthesis of nanomaterials. The biomineralization behavior and inorganic nanoparticle (NP) synthesis mechanism of (apo)ferritin in solution systems have been studied but the mineralization behavior of (apo)ferritin on the substrates has not yet been well studied. Here, we conducted quantitative and kinetic analyses of the mineralization behavior of immobilized (apo)ferritin on a polyelectrolyte multilayer (PEM) using quartz crystal microbalance (QCM), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS) techniques. We demonstrated that the (apo)ferritin immobilized on a substrate synthesizes a ferrihydrite core within the confines of the protein cage; similar to a solution dispersed system. In addition, we applied a ferritin/apoferritin blended monolayer to the study of iron mineralization and revealed that biomineralization in this system is spatially selective. It is important to understand the mineralization mechanisms for the synthesis of other functional NPs as this approach has potential for a broad range of magnetic, catalytic, and biomedical sensing applications.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Iron(III) oxide, powder, <5 μm, ≥96%
Sigma-Aldrich
Iron, ≥99%, reduced, powder (fine)
Sigma-Aldrich
Iron(III) oxide, nanopowder, <50 nm particle size (BET)
Sigma-Aldrich
Ferritin from equine spleen, Type I, saline solution
Sigma-Aldrich
Iron, powder, −325 mesh, 97%
Sigma-Aldrich
Carbonyl iron, ≥97% Fe basis
Sigma-Aldrich
Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)
Sigma-Aldrich
Iron, nanopowder, 35-45 nm particle size, 99.5% trace metals basis
Sigma-Aldrich
Iron, granular, 10-40 mesh, >99.99% trace metals basis
Sigma-Aldrich
Iron, chips, 99.98% trace metals basis
Sigma-Aldrich
Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Ferritin from human liver, Type IV, 10 μg/mL
Sigma-Aldrich
Iron, foil, thickness 0.25 mm, ≥99.99% trace metals basis
Sigma-Aldrich
Iron(III) oxide, dispersion, nanoparticles, ≤110 nm particle size, 15 wt. % in ethanol
Sigma-Aldrich
Ferritin from human spleen, Type V, 10 μg/mL in 0.15 M NaCl, 10 mM Tris, pH 8.0, containing 0.1% sodium azide
Sigma-Aldrich
Iron, wire, diam. 1.0 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Iron, carbon coated magnetic, nanopowder, 25 nm avg. part. size, 99.5% trace metals basis
Sigma-Aldrich
Iron, wire, diam. 0.5 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Iron, rod, diam. 6.3 mm, 99.98% trace metals basis
Iron, IRMM®, certified reference material, 0.5 mm wire
Iron, foil, 300x300mm, thickness 0.1mm, hard, 99.5%
Iron, foil, 0.2m coil, thickness 0.5mm, coil width 49mm, armco« soft ingot 99.8+%
Iron, wire reel, 1m, diameter 0.025mm, as drawn, 99.99+%
Iron, wire reel, 20m, diameter 0.125mm, hard, 99.5%
Iron, foil, 10mm disks, thickness 0.01mm, 99.85%
Iron, foil, 10mm disks, thickness 0.01mm, 99.99+%
Iron, foil, 10mm disks, thickness 0.020mm, 99.85%
Iron, foil, 10mm disks, thickness 0.025mm, as rolled, 99.99+%
Iron, foil, 10mm disks, thickness 0.025mm, hard, 99.5%
Iron, foil, 10mm disks, thickness 0.038mm, hard, 99.5%

Redes sociais

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Pesquisa. Desenvolvimento. Produção.

Somos um fornecedor global líder para o setor de ciências biológicas, com soluções e serviços para pesquisa, desenvolvimento e produção de biotecnologia, além de produção e desenvolvimento de terapias farmacológicas com medicamentos.

© 2021 Merck KGaA, Darmstadt, Alemanha e/ou suas filiais. Todos os direitos reservados.

A reprodução não autorizada de quaisquer materiais deste site é estritamente proibida.