HPLC Analysis of Aflatoxins in Peanut Paste on Ascentis® Express C18 following SPE using Supel™ Tox AflaZea
材料
付属品
CONDITIONS
sample preparation
Solid Phase Extraction
sample/matrix
raw peanut paste
SPE tube/cartridge
Supel Tox AflaZea, 6 mL (55314-U)
extraction process
suspend 25 g raw peanut paste in 100 mL acetonitrile:water (84:16) extraction solution
sample addition
directly load 2 mL extract onto SPE tube
elution
pull sample through the SPE tube by vacuum at a rate of 8 mL/min
eluate post-treatment
combine 200 μL eluate and 300 μL of water:trifluoroacetic acid:acetic acid (70:20:10 ) derivatization reagent, (heat at 65°C for 25 min, add 500 μL of water to derivatized sample and vortex 30 sec)
column
Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)
mobile phase
[A} acetonitrile; {B} water; (20:80, A:B)
flow rate
0.2 mL/min
column temp.
35 °C
detector
fluorescence, ex 365 nm, em 450 nm
injection
20 μL
sample
B1, G1 (8 ppb), B2, G2 (2 ppb)
詳細
アナリシスノート
Aflatoxins are carcinogenic metabolites produced by fungi that infect major agricultural commodites. The presence and concentrations of aflatoxins is widely regulated. Here, a cleanup method using interference removal SPE followed by analysis by HPLC with fluorescence detection is used to analyze derivatized samples extracted from raw peanut paste. The HPLC method was optimized for fluorescence detection and quantification of aflatoxins at < 10 ppb concentrations.
その他情報
Sample pretreatment: suspend 25 g raw peanut paste in 100 mL acetonitrile:water (84:16) extraction solution
法的情報
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC