The serum and plasma sample values for the HCYTA-60K kit are provided. It's important to note that these values should be used as a guideline only. This data is based on a small sample size without effort for randomization and is not a true reference range.
HCYTA-60K
MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A
Configurable Human Cytokine/Chemokine/Growth Factor 48-Plex Panel A
Synonym(s):
Human cytokine multiplex kit, Luminex® human cytokine immunoassay, Millipore human cytokine panel
Sign In to View Organizational & Contract Pricing.
To order a Milliplex® kit, please search for your analyte of interest.
About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Skip To
Product Name
MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A,
Quality Level
species reactivity
human
packaging
pkg of 1 ea
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
input
cell culture supernatant
serum
detection method
fluorometric (Luminex® xMAP® technology)
shipped in
wet ice
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | |||
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable |
| detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex® xMAP®) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
General description
Cytokines, chemokines, and growth factors are key mediatorsof immune system functions capable of signaling through autocrine, paracrine,and endocrine mechanisms. Their pleiotropic immunomodulatory propertiesallow these biomolecules to react to diverse stimuli and regulate the immuneresponse, either promoting or inhibiting inflammation.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 48 analytes in human serum, plasma, and cell culture supernatants.
Analytes included: sCD40L, EGF, Eotaxin/CCL11, FGF-2/FGF-basic, FLT-3L, Fractalkine/CX3CL1, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12(p70), IL-13, IL-15, IL-17A/CTLA8, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10/CXCL10, MCP-1/CCL2, MCP-3/CCL7, M-CSF, MDC, MIG/CXCL9, MIP-1α/CCL3, MIP-1β/CCL4, PDGF-AA, PDGF-AB/BB, RANTES/CCL5, TGFα, TNFα, TNFβ/LTA, VEGF-A
Note: RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.
Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Analytes included: sCD40L, EGF, Eotaxin/CCL11, FGF-2/FGF-basic, FLT-3L, Fractalkine/CX3CL1, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12(p70), IL-13, IL-15, IL-17A/CTLA8, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10/CXCL10, MCP-1/CCL2, MCP-3/CCL7, M-CSF, MDC, MIG/CXCL9, MIP-1α/CCL3, MIP-1β/CCL4, PDGF-AA, PDGF-AB/BB, RANTES/CCL5, TGFα, TNFα, TNFβ/LTA, VEGF-A
Note: RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.
Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Features and Benefits
- 48-Plex Power, Simplified: One kit unlocks 48 biomarkers—replaces multiple ELISAs and slashes costs.
- More Than 20-Year Multiplexing Legacy: The original multiplex innovator—trusted by top pharma companies for critical assays.
- Cancer, Autoimmunity, & Beyond: Power research in immunotherapy, sepsis, Alzheimer’s, and Long COVID biomarker discovery.
- Intuitive Workflow: User-friendly protocols that require minimal training, allowing researchers to achieve publication-ready results without extensive background in multiplex assays.
- Configurable Assay Design: Configure your panel by selecting specific analytes based on your research needs, allowing for a tailored approach that maximizes relevance and impact.
- Robust Sample Compatibility: Verified for serum, plasma, PBMC supernatants, and cell/tissue lysates. Tested across normal and disease-state samples for broad applicability.
- Peer-Reviewed Credibility: Frequently cited in immunology and clinical research publications. Trusted by academic institutions, CROs, and pharma companies.
- Performance Highlights from Comparative Studies: MILLIPLEX® assays showed highest reproducibility among Luminex®-based kits in a head-to-head study and in disease profiling (e.g., ovarian cancer, sepsis), and MILLIPLEX® assays detected more significantly elevated analytes than competitors.
- Global Support & Documentation: Backed by extensive technical documentation, protocols, and customer support.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
related product
Product No.
Description
Pricing
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
target_organs
Respiratory Tract
Storage Class
10 - Combustible liquids
wgk
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Carl A Pierce et al.
JCI insight, 6(9) (2021-04-07)
BACKGROUNDCoronavirus disease 2019 (COVID-19) is more benign in children compared with adults for unknown reasons. This contrasts with other respiratory viruses where disease manifestations are often more severe in children. We hypothesize that a more robust early innate immune response
Carl A Pierce et al.
medRxiv : the preprint server for health sciences (2021-02-18)
COVID-19 is more benign in children compared to adults for unknown reasons. This contrasts with viruses such as influenza where disease manifestations are often more severe in children1. We hypothesized that a more robust early innate immune response to SARS-CoV-2
Manuel Cabeza-Segura et al.
British journal of cancer, 127(12), 2198-2206 (2022-10-18)
Advanced gastro-oesophageal cancer (GEA) treatment has been improved by the introduction of immune checkpoint inhibitors (CPIs), yet identifying predictive biomarkers remains a priority, particularly in patients with a combined positive score (CPS) < 5, where the benefit is less clear. Our study
Yiwei Zhao et al.
Fertility and sterility, 115(4), 1044-1053 (2020-12-05)
To compare the changing peripheral levels of inflammation-related cytokine profile during a 9-day period after blastocyst transfer between women who did and did not conceive. Prospective, observational, and longitudinal study. University-affiliated hospital. Forty-seven women with infertility who were undergoing single
Liam J O'Neil et al.
Frontiers in immunology, 13, 958145-958145 (2022-09-27)
The development of autoantibody directed towards citrullinated proteins (ACPA) are predictive of RA in at-risk individuals. The biological events that underpin loss of immune tolerance and progression into inflammatory arthritis are not known. We sought to identify serum proteomic alterations
Related Content
MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panels A and B offer customizable options for analyzing nearly 100 cytokines.
Simplify protein biomarker discovery with the MILLIPLEX® PLEXpedition 115-Plex Multiplex Screening Panel. Effortlessly screen cytokines and other markers with Luminex® technology.
Encuentre los inmunoanálisis que permiten explorar las citocinas que intervienen en la neuroinmunología y la neuroinflamación, así como los biomarcadores de enfermedades neurodegenerativas.
Cytokine multiplex assays allow researchers to easily investigate the immune system. The MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Multiplex Panels A and B are multiplex assays for cytokine detection that each offer a unique combination of 48 analytes that can be simultaneously analyzed in a small sample volume.
-
Could you please provide the expected sample ranges for the Milliplex Human Cytokine Panel A (HCYTA-60K)?
1 answer-
Helpful?
-
-
Can the Milliplex Human Cytokine/Growth Factor Panel A (HCYTA-60K) measure IFNa2a or IFNA2B?
1 answer-
Based on the antibody and standard specification sheets, the kit detects both IFNa2a and IFNa2B. The capture antibody is listed as an IFNa2B antibody and does not mention IFNa2a. However, the standard is recombinant IFNa2a.
Helpful?
-
-
Could you provide a recommendation for sample preparation for placenta and HCYTA-60K assay?
1 answer-
The Milliplex assays have not been tested or verified for use with placenta tissue homogenates. However, a reference is available using placenta homogenates in the Human Cytokine Panel 1 (HCYTOMAG-60K).
The samples were prepared as follows:
Placental homogenate: Fragments of frozen placental tissue were transferred to propylene tubes containing lysis buffer and a cocktail of protease inhibitors. The placenta samples were homogenized using an electric homogenizer on ice. After homogenization, the homogenates were centrifuged, and the protein concentration in the supernatant was measured and stored for further analysis.Reference: Molás RB, Ribeiro MR, Ramalho Dos Santos MJC, et al. The involvement of annexin A1 in human placental response to maternal Zika virus infection Antiviral Res. 2020 Jul; 179:104809.
Helpful?
-
-
Are the standards used in Human Cytokine Panel A expressed in E. Coli?
1 answer-
The IFNa2 protein is expressed in yeast, while IL-12p40, IL-12p70, and IL-27 proteins are expressed in HEK293. All the other proteins are expressed in E. Coli.
Helpful?
-
-
What type of IL-12p40 was detected in the Human Cytokine Panel A (HCYTA-60K) – IL-12p40 monomer or IL-12p40 total (both monomer and homodimer)?
1 answer-
The capture in the assay detects the IL-12p40 monomer, and the standard used is also the IL-12p40 monomer. However, specific testing has not been conducted to determine if it also detects the homodimer.
Helpful?
-
-
When precipitating proteins (albumin and globulins) from plasma or serum samples using an Acetonitrile solution, does cortisol, which is bound to albumin & globulin, dissociate into the solution or get removed along with the protein precipitate?
1 answer-
The treatment with acetonitrile removes the matrix effect, including the proteins, from the samples. This means that the cortisol bound to albumin and other proteins is eliminated. The assay specifically measures the free cortisol (non-bound cortisol).
Helpful?
-
-
Is it better to use EDTA vacutainers than heparinized vacutainers when collecting blood from plasma? Does this apply to both humans and mice?
1 answer-
For our Milliplex assays, EDTA plasma is preferred. Care must be taken when using heparin as an anticoagulant since an excess of heparin will provide falsely high values. Use no more than 10 IU heparin per mL of blood collected.
Helpful?
-
-
Do you have any information about BPA, specifically bisphenol A from the plastic used in the immunoassay kits?
1 answer-
The plastic materials used in the immunoassay kit are BPA-free according to the manufacturer's websites.
Helpful?
-
-
Is there an alternative method if a bath sonicator is not available for sonication of beads as mentioned in the Milliplex protocols? Can vortexing for a longer period serve as an alternative?
1 answer-
The Milliplex assays recommend using a sonicator (water bath, Branson B200 or equivalent). Sonication helps break up any aggregated beads and beads stuck to the vial to reduce aggregation and improve counts. If a bath sonicator is not available, you can vortex for up to 2 minutes.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service