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MilliporeSigma

A1420

Ames′ Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

NACRES:
NA.75
UNSPSC Code:
41161501

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Product Name

Ames′ Medium, With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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This Item
M8403W0397M4892
Sigma-Aldrich

Sigma-Aldrich

A1420

Ames′ Medium

Sigma-Aldrich

Sigma-Aldrich

M8403

McCoy′s 5A Medium

SAFC

SAFC

W0397

Williams′ Medium E

SAFC

SAFC

M4892

McCoy′s 5A Medium

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

liquid

form

liquid

form

powder

components

glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L

components

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

components

NaHCO3: 2.2 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
sodium pyruvate: 0.025 g/L
L-glutamine: 0.292 g/L

components

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

retinal tissue storage maintenance

classic media, classical media, FBS, primary cell culture, virus production

classic media, classical media, FBS

classic media, classical media, FBS, primary cell culture, vaccine production media

Application

Ames′ Medium has been used to maintain rat/mice/macaque monkey eye for the isolation of retina.[1][2][3]

General description

Ames′ Medium has been formulated to support retinal tissue in relatively short-term culture. It is suitable for maintaining central nervous system tissue in vitro.

Preparation Note

Formulated to contain 8.8 grams of powder per liter of medium.
Supplement with 1.9 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000451376
0000451376
24M407
24M407
0000416084

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B Ameline et al.
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Several preclinical studies have investigated the potential of algal channelrhodopsin and human melanopsin as optogenetic tools for vision restoration. In the present study, we assessed the potentially deleterious effects of long-term expression of these optogenes on the diseased retina in
John M Nagarah et al.
Frontiers in neuroscience, 9, 384-384 (2015-11-06)
Multielectrode arrays (MEAs) allow for acquisition of multisite electrophysiological activity with submillisecond temporal resolution from neural preparations. The signal to noise ratio from such arrays has recently been improved by substrate perforations that allow negative pressure to be applied to
Sabrina Asteriti et al.
Visual neuroscience, 34, E006-E006 (2017-10-03)
Rod-cone gap junctions mediate the so-called "secondary rod pathway", one of three routes that convey rod photoreceptor signals across the retina. Connexin 36 (Cx36) is expressed at these gap junctions, but an unidentified connexin protein also seems to be expressed.
Michael B Manookin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(16), 4136-4150 (2008-04-18)
Cone signals divide into parallel ON and OFF bipolar cell pathways, which respond to objects brighter or darker than the background and release glutamate onto the corresponding type of ganglion cell. It is assumed that ganglion cell excitatory responses are
Qiuju Jiang et al.
IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society, 26(5), 969-976 (2018-05-13)
Significant progress has been made recently in treating neurological blindness using implantable visual prostheses. However, implantable medical devices are highly invasive and subject to many safety, efficacy, and cost issues. The discovery that ultrasound (US) may be useful as a
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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