P2545

Protein A–Agarose

Name: Protein A–Agarose
Brand: SIGMA
Price: 245.65 USD
Availability: InStock

saline suspension

Synonym(s):

Protein A resin

Slide 1 of 1

Select a Size

1 ML

$245.65

5 ML

$802.40

$245.65

List Price$289.00Save 15%

Available to ship TODAYDetails

About This Item

UNSPSC Code:

41106500

NACRES:

NA.56

MDL number:

MFCD00165563

Key Documents

View All Documentation

Product Name

Protein A–Agarose, saline suspension

biological source

Staphylococcus aureus

form

saline suspension

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Antibody Protein Purification

Protein Biology

Protein Purification Chromatography

Protein Sample Prep

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	P9269	P7155	P5906
Millipore P2545 Protein A–Agarose	Millipore P9269 Protein A–Agarose	Sigma-Aldrich P7155 Protein A from Staphylococcus aureus	Millipore P5906 Protein A (extracellular)–Agarose from Staphylococcus aureus
biological source Staphylococcus aureus	biological source -	biological source -	biological source Staphylococcus aureus
form saline suspension	form lyophilized powder	form cell suspension	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
extent of labeling ~2 mg per mL	extent of labeling ~2 mg per mL	extent of labeling -	extent of labeling ~2 mg per mL
matrix activation cyanogen bromide	matrix activation cyanogen bromide	matrix activation -	matrix activation cyanogen bromide

Application

Protein A−Agarose has been used in the immunoprecipitation

of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells[1]
of prolyl hydroxylase domain 2 N27 cells[2]
of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells[3]

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG.[4] Protein A agarose resin is commercially used for affinity based purifications.[5]

General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.[4]

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Deficiency of antibody responses to T-independent antigens in gerbils---Meriones unguiculatus.

Madhu Chhanda Mohanty et al.

Developmental and comparative immunology, 26(4), 385-391 (2002-03-13)

Meriones unguiculatus commonly known as gerbils are widely used as animal models for a variety of parasitic infections such as Brugia malayi, Entamoeba histolytica, Giardia duodenalis, Toxoplasma gondi, Helicobacter pylori, Strongyloides stercoralis and Echinococcus multilocularis. Groups of BALB/c mice, gerbils

Roles of calreticulin and calnexin during mucin synthesis in LS180 and HT29/A1 human colonic adenocarcinoma cells.

D J McCool et al.

The Biochemical journal, 341 ( Pt 3), 593-600 (1999-07-27)

Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to look for possible involvement of the chaperones calreticulin (CRT) and calnexin

Evaluation of a novel methacrylate-based protein a resin for the purification of immunoglobulins and Fc-fusion proteins

McCaw TR, et al.

Biotechnology Progress, 30(5), 1125-1136 (2014)

Hsp90 Co-chaperone p23 contributes to dopaminergic mitochondrial stress via stabilization of PHD2: Implications for Parkinson?s disease

Rane A, et al.

Neurotoxicology, 65, 166-173 (2018)

PTEN-Dependent Stabilization of MTSS1 Inhibits Metastatic Phenotype in Pancreatic Ductal Adenocarcinoma

Zeleniak AE, et al.

Neoplasia, 20(1), 12-24 (2018)

View All Related Papers

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Protein A–Agarose