MilliporeSigma
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P2545

Millipore

Protein A–Agarose

saline suspension

All Photos(1)
Synonym(s):
Protein A resin
MDL number:
MFCD00165563
NACRES:
NA.56

biological source

Staphylococcus aureus

Quality Level

200

form

saline suspension

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

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Protein A–Agarose

Protein A–Agarose lyophilized powder

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P1925

Protein A–Agarose macrobeads

Protein A–Agarose lyophilized powder

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P1406

Protein A–Agarose

biological source

Staphylococcus aureus

biological source

-

biological source

-

biological source

-

form

saline suspension

form

lyophilized powder

form

aqueous suspension

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

extent of labeling

~2 mg per mL

extent of labeling

~2 mg per mL

extent of labeling

~6 mg per mL

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Application

Protein A−Agarose has been used in the immunoprecipitation
  • of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
  • of prolyl hydroxylase domain 2 N27 cells
  • of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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