MilliporeSigma
All Photos(1)

P2545

Millipore

Protein A–Agarose

saline suspension

Synonym(s):
Protein A resin
MDL number:
NACRES:
NA.56

biological source

Staphylococcus aureus

Quality Level

form

saline suspension

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

≥15 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

1 of 4

This Item
P9269P1925P1406
Millipore

Millipore

P2545

Protein A–Agarose

Protein A–Agarose lyophilized powder

Millipore

P9269

Protein A–Agarose

Protein A–Agarose lyophilized powder

Millipore

P1406

Protein A–Agarose

biological source

Staphylococcus aureus

biological source

-

biological source

-

biological source

-

form

saline suspension

form

lyophilized powder

form

aqueous suspension

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

extent of labeling

~2 mg per mL

extent of labeling

~2 mg per mL

extent of labeling

~6 mg per mL

extent of labeling

~2 mg per mL

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

General description

Protein A is a 42-kDa protein from Staphylococcus aureus and has five immunoglobulin binding domains.

Application

Protein A−Agarose has been used in the immunoprecipitation
  • of protein phosphatase and tensin homolog (PTEN) protein from S63 and NIH3T3 fibroblast cells
  • of prolyl hydroxylase domain 2 N27 cells
  • of Wnt3a and sonic hedgehog (Shh) in mouse fibroblast L-cells and human embryonic kidney (HEK) 293 cells

Protein A-agarose is used for affinity chromatography, antibody purification and characterization, and protein A, G and L resins. Protein A-agarose has been used to study the effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy as well as to study multiple sclerosis and gastric cancer.

Biochem/physiol Actions

Protein A binds to Fcγ (the constant region) and Fab (antigen binding fragment) of IgG. Protein A agarose resin is commercially used for affinity based purifications.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

(enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service