X4126

Xylene Cyanol FF

BioReagent, Molecular Biology

• Synonym(s): Acid blue 147, Cyanol FF, XC
• Empirical Formula (Hill Notation): C₂₅H₂₇N₂NaO₆S₂
• CAS Number: 2650-17-1
• Molecular Weight: 538.61
• UNSPSC Code: 12171500
• NACRES: NA.52
• PubChem Substance ID: 57654732
• EC Number: 220-167-5
• Colour Index Number: 42135
• MDL number: MFCD00019481

Properties

• Product Name: Xylene Cyanol FF, BioReagent, Molecular Biology
• InChI key: VVLFAAMTGMGYBS-KRQUPCAFSA-M
• InChI: 1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;
• SMILES string: [Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O
• grade: Molecular Biology
• product line: BioReagent
• form: powder
• technique(s): nucleic acid detection: suitable
• solubility: H₂O: complete 1 mg/mL, clear, blue
• suitability: suitable for nucleic acid staining
• storage temp.: room temp
• Quality Level: 200

Description

Application

Xylene Cyanol FF is used as a:

• histological stain and as tracker dye in DNA sequencing and electrophoresis[1][2]
• gel-loading dye[3]
• formamide RNA loading dye[4]
• tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer[5]

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves