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X4126

Sigma-Aldrich

Xylene Cyanol FF

for molecular biology, BioReagent

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Synonym(s):
Acid blue 147, Cyanol FF, XC
Empirical Formula (Hill Notation):
C25H27N2NaO6S2
CAS Number:
Molecular Weight:
538.61
Colour Index Number:
42135
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

product line

BioReagent

form

powder

technique(s)

nucleic acid detection: suitable

solubility

H2O: complete 1 mg/mL, clear, blue

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O

InChI

1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;

InChI key

VVLFAAMTGMGYBS-KRQUPCAFSA-M

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This Item
B3269335940205486
Xylene Cyanol FF for molecular biology, BioReagent

X4126

Xylene Cyanol FF

Xylene Cyanol FF Dye content ≥75 %

335940

Xylene Cyanol FF

Xylenol Blue indicator grade, Dye content 90 %

205486

Xylenol Blue

grade

for molecular biology

grade

-

grade

-

grade

indicator grade

technique(s)

nucleic acid detection: suitable

technique(s)

-

technique(s)

-

technique(s)

titration: suitable

solubility

H2O: complete 1 mg/mL, clear, blue

solubility

water: 0.2 vial/mL, blue

solubility

water: 1 mg/mL, blue

solubility

-

form

powder

form

lyophilized powder

form

powder or crystals

form

powder

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Xylene Cyanol FF is used as a:
  • histological stain and as tracker dye in DNA sequencing and electrophoresis
  • gel-loading dye
  • formamide RNA loading dye
  • tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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