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MilliporeSigma

M4159

Methylene Blue hydrate

suitable for nucleic acid staining, BioReagent

Synonym(s):

3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
122965-43-9
Molecular Weight:
319.85 (anhydrous basis)
Beilstein/REAXYS Number:
3599847
EC Number:
200-515-2
MDL number:
MFCD00150006
UNSPSC Code:
12171500
PubChem Substance ID:
57654386
NACRES:
NA.52

Key Documents

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grade

Molecular Biology

product line

BioReagent

form

powder

technique(s)

nucleic acid detection: suitable

solubility

water: 1 mg/mL (dark blue)

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

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This Item
28514M914066719
Methylene Blue hydrate suitable for nucleic acid staining, BioReagent

Sigma-Aldrich

M4159

Methylene Blue hydrate

Methylene Blue hydrate ≥95% (calc. to the dried substance)

Sigma-Aldrich

28514

Methylene Blue hydrate

Methylene blue certified by the BSC

Sigma-Aldrich

M9140

Methylene blue

Methylene Blue hydrate meets analytical specification of BP 73

Sigma-Aldrich

66719

Methylene Blue hydrate

technique(s)

nucleic acid detection: suitable

technique(s)

-

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

grade

for molecular biology

grade

-

grade

certified by the Biological Stain Commission

grade

-

suitability

suitable for nucleic acid staining

suitability

-

suitability

-

suitability

-

solubility

water: 1 mg/mL (dark blue)

solubility

ethanol: insoluble ≤1%

solubility

H2O: 4mg/4ml

solubility

-

form

powder

form

powder

form

powder

form

powder

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

-

General description

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

Application

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay.[1] Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.[2]

Features and Benefits

  • Less hazardous than staining with ethidium bromide
  • Viewable in visible light
  • Fast and easy to use

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT1552
SHBS6805
SHBS3531
SHBQ6330
SHBQ0906

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Angela L Davis et al.
International journal of molecular sciences, 14(2), 4185-4202 (2013-02-23)
Repurposing approved and abandoned non-oncological drugs is an alternative developmental strategy for the identification of anticancer therapeutics that has recently attracted considerable attention. Due to the essential role of the cellular heat shock response in cytoprotection through the maintenance of
Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.
A C Peacock et al.
Biochemistry, 6(6), 1818-1827 (1967-06-01)
Young-Sharp, D. and Kumar, R.
Technique, 1, 183-183 (1989)
Fei Yan et al.
Photochemistry and photobiology, 86(3), 662-666 (2010-02-06)
This study reports that photosensitizers encapsulated in supramolecular protein cages can be internalized by tumor cells and can deliver singlet oxygen intracellularly for photodynamic therapy (PDT). As an alternative to other polymeric and/or inorganic nanocarriers and nanoconjugates, which may also
Domenic Di Paola et al.
Nucleic acids research, 38(7), 2314-2331 (2010-01-13)
Using libraries of replication origins generated previously, we identified three clones that supported the autonomous replication of their respective plasmids in transformed, but not in normal cells. Assessment of their in vivo replication activity by in situ chromosomal DNA replication
View All Related Papers

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