796-0658

HPLC Analysis of Proteins on Discovery^® BIO PolyMA-SCX (Analyte Set #2)

application for HPLC

• Synonym(s): Proteins, mixture

Properties

• description: Separated on Discovery^® BIO PolyMA-SCX
• technique(s): HPLC: suitable
• test parameters: column: Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U); mobile phase: [A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0; 5 to 100% B in 15 min. (linear); flow rate: 0.5 mL/min; injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A; detector: UV, 280 nm
• suitability: application for HPLC
• application(s): life science and biopharma

Description

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the power of ion-exchange to resolve proteins with very little difference in molecular weight, an advantage over size-exclusion separations.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany