• Home
  • Search Results
  • Specificity of the horizontal cell-photoreceptor connections in the zebrafish (Danio rerio) retina.

Specificity of the horizontal cell-photoreceptor connections in the zebrafish (Danio rerio) retina.

The Journal of comparative neurology (2009-08-06)
Yong N Li, Jonathan I Matsui, John E Dowling
ABSTRACT

Horizontal cells (HCs) are involved in establishing the center-surround receptive field organization of photoreceptor and bipolar cells. In many species, HCs respond differentially to colors and may play a role in color vision. An earlier study from our laboratory suggested that four types of HCs exist in the zebrafish retina: three cone HCs (H1, H2 and H3) and one rod HC. In this study, we describe their photoreceptor connections. Cones are arranged in a mosaic in which rows of alternating blue (B)- and ultraviolet (UV)-sensitive single cones alternate with rows of red (R)- and green (G)-sensitive double cones; the G cones are adjacent to UV cones and B cones adjacent to R cones. Two small-field (H1 and H2) and two large-field (H3 and rod HC) cells were observed. The cone HC dendritic terminals connected to cones with single boutons, doublets, or rosettes, whereas the rod HCs connected to rods with single boutons. The single boutons/doublets/rosettes of cone HCs were arranged in double rows separated by single rows for H1 cells, in pairs and singles for H2 cells, and in a rectilinear pattern for H3 cells. These connectivity patterns suggest that H1 cells contact R, G, and B cones, H2 cells G, B, and UV cones, and H3 cells B and UV cones. These predictions were confirmed by applying the DiI method to SWS1-GFP retinas whose UV cones express green fluorescent protein. Each rod HC was adjacent to the soma or axon of a DiI-labeled cone HC and connected to 50-200 rods.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phosphate buffered saline, tablet
Sigma-Aldrich
Phosphate buffered saline, powder, pH 7.4, for preparing 1 L solutions
Sigma-Aldrich
Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture
Sigma-Aldrich
Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4
Sigma-Aldrich
Phosphate buffered saline, BioPerformance Certified, pH 7.4
Sigma-Aldrich
Phosphate Buffered Saline, 10× PBS for Western blots and IP
Sigma-Aldrich
Phosphate buffered saline, powder, pH 7.4, for preparing 5 L solutions
Sigma-Aldrich
Phosphate buffered saline, pH 7.2 (25 °C)
Sigma-Aldrich
Phosphate buffered saline, BioUltra, pH 7.4 ( in solution), contains TWEEN 20, tablet
Sigma-Aldrich
Phosphate buffered saline, pH 7.4, contains BSA, powder
Sigma-Aldrich
Phosphate buffered saline, BioUltra, washing buffer for peroxidase conjugates in Western Blotting, 10x concentrate
Sigma-Aldrich
Phosphate buffered saline, BioUltra, solution
Sigma-Aldrich
Phosphate Buffered Saline with 3% Non-Fat Milk, pH 7.4
Sigma-Aldrich
Phosphate buffered saline, Autoclaved, pH 7.2 (25 °C)
Sigma-Aldrich
Phosphate buffered saline, with 5% nonfat milk, powder (dry milled), pH 7.3
Sigma-Aldrich
Phosphate buffered saline, pH 7.6 (25 °C)