• Home
  • Search Results
  • Toll-like receptor 4-dependent responses to lung injury in a murine model of pulmonary contusion.

Toll-like receptor 4-dependent responses to lung injury in a murine model of pulmonary contusion.

Shock (Augusta, Ga.) (2008-07-31)
J Jason Hoth, Jonathan D Wells, Noel A Brownlee, Elizabeth M Hiltbold, J Wayne Meredith, Charles E McCall, Barbara K Yoza
ABSTRACT

Blunt chest trauma resulting in pulmonary contusion with an accompanying acute inflammatory response is a common but poorly understood injury. We previously demonstrated that toll-like receptor 2 (TLR-2) participates in the inflammatory response to lung injury. We hypothesized that the TLR-4, in an MyD88-dependent manner, may also participate in the response to lung injury. To investigate this, we used a model of pulmonary contusion in the mouse that is similar to that observed clinically in humans and evaluated postinjury lung function, pulmonary neutrophil recruitment, and the systemic innate immune response. Comparisons were made between wild-type mice and mice deficient in TLR-4 or MyD88. We found TLR-4-dependent responses to pulmonary contusion that include hypoxemia, edema, and neutrophil infiltration. Increased expression of IL-6 and chemokine (C-X-C motif) ligand 1 in the bronchoalveolar lavage and serum was also dependent on TLR-4 activation. We further demonstrated that these responses to pulmonary contusion were dependent on MyD88, an adapter protein in the signal transduction pathway mediated by TLRs. These results show that TLRs have a primary role in the response to acute lung injury. Lung inflammation and systemic innate immune responses are dependent on TLR activation by pulmonary contusion.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phosphate buffered saline, tablet
Sigma-Aldrich
Phosphate buffered saline, powder, pH 7.4, for preparing 1 L solutions
Sigma-Aldrich
Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture
Sigma-Aldrich
Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4
Sigma-Aldrich
Phosphate buffered saline, BioPerformance Certified, pH 7.4
Sigma-Aldrich
Phosphate Buffered Saline, 10× PBS for Western blots and IP
Sigma-Aldrich
Hematoxylin Solution, Mayer′s
Sigma-Aldrich
Hematoxylin Solution, Mayer′s
Sigma-Aldrich
Phosphate buffered saline, powder, pH 7.4, for preparing 5 L solutions
Sigma-Aldrich
Phosphate buffered saline, pH 7.2 (25 °C)
Sigma-Aldrich
Hematoxylin solution according to Mayer
Sigma-Aldrich
Phosphate buffered saline, BioUltra, pH 7.4 ( in solution), contains TWEEN 20, tablet
Sigma-Aldrich
Hematoxylin Solution, Mayer′s
Sigma-Aldrich
Phosphate buffered saline, pH 7.4, contains BSA, powder
Sigma-Aldrich
Phosphate buffered saline, BioUltra, washing buffer for peroxidase conjugates in Western Blotting, 10x concentrate
Sigma-Aldrich
Phosphate buffered saline, BioUltra, solution
Sigma-Aldrich
Phosphate Buffered Saline with 3% Non-Fat Milk, pH 7.4
Sigma-Aldrich
Phosphate buffered saline, Autoclaved, pH 7.2 (25 °C)
Sigma-Aldrich
Phosphate buffered saline, with 5% nonfat milk, powder (dry milled), pH 7.3
Sigma-Aldrich
Hematoxylin Solution, Mayer′s
Sigma-Aldrich
Phosphate buffered saline, pH 7.6 (25 °C)
Sigma-Aldrich
Hematoxylin Solution, Mayer′s