Protein Extraction, Purification, Quantitation, & Analysis Techniques

Protein Research

Extraction, isolation, purification, separation, detection—we know your time is better spent analyzing data than performing arduous protocols before you can even measure your proteins of interest. We can help with the most efficient approaches to cloning and expression with a wide variety of cells, reagents, and vectors. Our membrane engineers have developed devices expressly for desalting, concentrating, and buffer exchange that save your proteins, and save you time and protocol steps. A comprehensive offering of protein preparation products provide gentle extraction, high-yield isolation and purification, and application-verified detection tools for techniques like electrophoresis and Western blot, ELISA, multiplex, and mass spectrometry.

Stabilyser™ Protein & RNA Stabilization Reagent

Immobilon®-E Membrane


 Key Applications for Protein Research

 

Protein Expression
Find the best system for recombinant expression.
• Competent bacteria
• Yeast expression systems
• Vectors
• Competent cells
• Transformation/transfection reagents

 

Lysis & Protein Extraction
Detergents and protease inhibitors to maximize cell lysis efficiency and obtain the high protein yield.
• Busters
• Benzonase
• Inhibitor cocktails
Roche cOmplete™ protease inhibitors

 

Protein Purification & Isolation
Agarose resins & magnetic beads for diverse protein purification strategies.
• Immunoprecipitation (IP)
Recombinant Protein Purification
Affinity Purification
Protein Sample Purification
 

 

Protein Concentration & Buffer Exchange
Fast and gentle sample concentration, buffer exchange, desalting and dialysis.
Amicon® Ultra centrifugal filters
Large volume concentration
D-tube dialyzers

 

Separation/Gel Electrophoresis

Precast gels, buffers & solvents, and prestained molecular weight markers to improve protein resolution with SDS-PAGE.

 

 

Western Blotting

Get the right band when you use tools optimized for your target protein.
• Transfer membranes
• Blocking buffers
• Detection reagents
Western blotting protocol

 

Antibodies
Over 90,000 world-class primary and secondary antibodies, conjugates and technical resources.




 

 

Protein Assays & Analysis
Accurately interrogate the proteins relevant to your system.
Duolink® PLA protein detection
  technology

ELISA
MILLIPLEX® multiplex assays using
  Luminex® technology

SMC (single molecule counting) assay

 

Proteomics & Mass Spectrometry
The expanding field of proteomics requires high-quality mass spec reagents.
MS internal standards
Seppro Protein Depletion
Proteomics Core
Glycoprotein Analysis


 

Protein Quantitation
Trusted methods for quantitation including BCA, Bradford, FluoroProfile




 

 

Core Protein Reagents
Essential reagents and tools to support your protein research.
Detergents
Enzymes and Proteins
Membrane protein reagents
General protease inhibitors
Reagents for protein stabilization

 

Life Science Labware & Equipment
From mixing and storage to separation and transfer, we offer:
Tissue homogenizers
Electrophoresis supplies
Glassware and Labware
Centrifugation supplies
Filtration supplies