T1503

Trizma^® base

Primary Standard and Buffer, ≥99.9% (titration), crystalline

• Sinônimo(s): Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
• Fórmula linear: NH₂C(CH₂OH)₃
• Número CAS: 77-86-1
• Peso molecular: 121.14
• Beilstein: 741883
• Número EC: 201-064-4
• Número MDL: MFCD00004679
• ID de substância PubChem: 24899973
• NACRES: NA.25

Propriedades

• Nível de qualidade: 300; 400
• descrição: aminopeptidase substrate
• teor: ≥99.9% (titration)
• forma: crystalline
• pH: 10.5-12
• useful pH range: 7-9
• pKa (25 °C): 8.1
• pb: 219-220 °C/10 mmHg (lit.)
• pf: 167-172 °C (lit.)
• solubilidade: methanol: soluble 26 mg/mL at 25 °C; ethylene glycol: soluble 79.1 mg/mL at 25 °C; water: soluble (678 g/l at 20 °C)
• absorção: ≤0.05 at 290 nm at 40%
• application(s): diagnostic assay manufacturing
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Descrição

Descrição geral

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

40% (w/w) solution in water is clear and colorless.

Aplicação

Trizma^® base has been used:

• as a component of H buffer (cell dissociation buffer)
• for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
• as an assay buffer for reconstitution of extracted and dried protein samples
• to prepare Tris-HCl buffer that is used to stabilize proteins
• as a buffer to extract carotenoid from tubers
• as a component of sample buffer during protein extraction prior to western blotting
• as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
• to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
• as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate

Embalagem

5, 10, 25, 50 kg in poly drum

25, 100, 250, 500 g in poly bottle

1 kg in poly bottle

Outras notas

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Easily compare specifications for Trizma products with the Trizma specification table.

Informações legais

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• WGK: WGK 1
• Ponto de fulgor (ºF): Not applicable
• Ponto de fulgor (ºC): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves