Merck
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T1503

Sigma-Aldrich

Trizma® base

Primary Standard and Buffer, ≥99.9% (titration), crystalline

Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.25

Nível de qualidade

descrição

aminopeptidase substrate

teor

≥99.9% (titration)

forma

crystalline

pH

10.5-12

useful pH range

7-9

pKa (25 °C)

8.1

pb

219-220 °C/10 mmHg (lit.)

pf

167-172 °C (lit.)

solubilidade

methanol: soluble 26 mg/mL at 25 °C
ethylene glycol: soluble 79.1 mg/mL at 25 °C
water: soluble (678 g/l at 20 °C)

absorção

≤0.05 at 290 nm at 40%

application(s)

diagnostic assay manufacturing

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Descrição geral

40% (w/w) solution in water is clear and colorless.
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Aplicação

Trizma® base has been used:
  • as a component of H buffer (cell dissociation buffer)
  • for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique
  • as an assay buffer for reconstitution of extracted and dried protein samples
  • to prepare Tris-HCl buffer that is used to stabilize proteins
  • as a buffer to extract carotenoid from tubers
  • as a component of sample buffer during protein extraction prior to western blotting
  • as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
  • to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
  • as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate

Outras notas

Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Informações legais

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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