This DMSO product has not been tested for suitability in HPLC applications. Please see the links below to review DMSO products suitable for HPLC:
https://www.sigmaaldrich.com/product/sigald/34869
https://www.sigmaaldrich.com/product/saj/074871
https://www.sigmaaldrich.com/product/sial/94563
D2650
ジメチルスルホキシド
Hybri-Max™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Dimethyl sulfoxide
別名:
DMSO, メチルスルホキシド
ログイン 組織・契約価格を表示する
サイズを選択してください
100 ML
￥31,520
￥31,520
単価￥39,400割引20%本日発送予定詳細
この商品について
化学式:
(CH3)2SO
CAS番号:
分子量:
78.13
Beilstein:
506008
EC Number:
MDL番号:
UNSPSCコード:
12191502
PubChem Substance ID:
NACRES:
NA.75
Bp:
189 °C (lit.)
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グレード
Hybri-Max™
品質水準
蒸気密度
2.7 (vs air)
蒸気圧
0.42 mmHg ( 20 °C)
無菌性
sterile-filtered
製品種目
BioReagent
アッセイ
≥99.7%
フォーム
liquid
自己発火温度
573 °F
expl. lim.
42 %, 63 °F
テクニック
cell culture | hybridoma: suitable
cryopreservation: suitable
不純物
endotoxin, tested
≤0.1% water
water
屈折率
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
溶解性
H2O: miscible (completely)
密度
1.10 g/mL (lit.)
SMILES記法
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI Key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
類似した製品をお探しですか？ 訪問 製品比較ガイド
1 of 4
当該品目 | D2653 | D2438 | V900090 |
|---|---|---|---|
| technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) cell culture | hybridoma: suitable, cryopreservation: suitable | technique(s) - |
| Quality Level 400 | Quality Level 300 | Quality Level 400 | Quality Level - |
| grade Hybri-Max™ | grade Hybri-Max™ | grade BioPerformance Certified | grade reagent grade |
| assay ≥99.7% | assay ≥99.7% | assay - | assay 99% |
| solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility H2O: miscible (completely) | solubility - |
| form liquid | form liquid | form liquid | form liquid |
アプリケーション
DMSOは極性非プロトン性溶媒であり、化学反応、ポリメラーゼ連鎖反応(PCR)や細胞、組織、臓器の透過型凍結保護試薬として使用されます。また、機械的損傷を引き起こす氷晶から細胞を保護する目的で、細胞の凍結培地に使用されます。初代培養、継代培養、組み換えによる異数体やハイブリドーマ細胞株をはじめ胎性幹細胞(ESC)、造血幹細胞の凍結保存に利用されます。DMSOは多くの場合、BSAやウシ胎児血清(FBS)とともに使用されます。
その他情報
本製品はHybri-Maxの製品です。ハイブリドーマ試験済みで、細胞凍結の適合性についても評価済みです。本製品は滅菌濾過済み(0.2ミクロン)で、エンドトキシンレベルについても検査済みです。
法的情報
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
免責事項
容易に過冷却状態となり、室温で徐々に再融解します。凝固している場合は室温に放置すると、劣化させることなく液体に戻すことができます。
used together
関連製品
製品番号
詳細
価格
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
188.6 °F - closed cup
引火点(℃)
87 °C - closed cup
個人用保護具 (PPE)
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
消防法
第4類:引火性液体
第三石油類
危険等級III
水溶性液体
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
D2650-1L:4548173193991
D2650-5X10ML:4548173194011
D2650-5X5ML:4548173194028
D2650-100ML:4548173193984
D2650-500ML:4548173194004
D2650-VAL:
D2650-PROC:
D2650-100ML-C:
D2650-VAR:
D2650-BULK:
D2650-5X5ML-C:
Azadeh Ghaee et al.
Materials science & engineering. C, Materials for biological applications, 103, 109767-109767 (2019-07-28)
In this study, a simple one-step method was introduced to prepare suitable PCL wound dressing by using a small amount of hydrophilic surface modifying macromolecule (LSMM) with ends capped with Polyethylene glycol and aminated zeolitic imidazolate frameworks (MZIF-8) as a
Stepan Coufal et al.
Cells, 8(7) (2019-07-25)
Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of inflammatory bowel disease (IBD)-gut dysbiosis, gut barrier failure and immune system dysregulation. While clinical differences among them
Hongxiu Diao et al.
BMC veterinary research, 15(1), 276-276 (2019-08-04)
Mammary gland tumor is the most common spontaneous tumor in intact female dogs, and its poor prognosis remains a clinical challenge. Ivermectin, a well-known anti-parasitic agent, has been implicated as a potential anticancer agent in various types of human cancer.
Likun Gao et al.
Cell death & disease, 10(8), 609-609 (2019-08-14)
Paeonol (Pae), a phenolic acid compound isolated from the Moutan Cortex, was previously demonstrated to exert multiple anticancer effects. The rational control of autophagy has been considered a potential treatment strategy for epithelial ovarian cancer. However, whether Pae induces autophagy
Shady Sayed et al.
Methods (San Diego, Calif.), 164-165, 36-48 (2019-05-13)
The CRISPR/Cas9 system is transforming many biomedical disciplines, including cancer research. Through its flexible programmability and efficiency to induce DNA double strand breaks it has become straightforward to introduce cancer mutations into cells in vitro and/or in vivo. However, not
資料
Overview of good cell banking practices for cell line cryopreservation purposes.
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
プロトコル
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
関連コンテンツ
Product Information Sheet
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Is this DMSO suitable for HPLC?
1 回答-
役に立ちましたか？
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Is this DMSO suitable to be used as a drug solvent in animal studies?
1 回答-
This product has not been tested for use in animal models. However, this is a sterile-filtered DMSO that has been tested for cytotoxicity and is expected to be suitable for in vivo research applications.
役に立ちましたか？
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How many days after opening the bottle is DMSO safe to use?
1 回答-
This product is assigned an expiration date of approximately two years from the date of Quality Release. This date is lot specific and available on the product label and Certificate of Analysis. After opening, the material should be stored tightly sealed to prevent moisture uptake. Given proper handling and storage, the product should be stable until the published expiration date.
役に立ちましたか？
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I ordered 10 x 10ml vials for fibroblast culture work. My first endeavor in culture domain. What are the storage conditions for DMSO of this grade? This arrived in plain cardboard box at room temperature.
1 回答-
DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots with inert gas and store them in a compatible, tightly sealed container, at a cool storage area, such as 4°C. DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Safety Data Sheet and the Product Information Sheet for compatibility of DMSO with various materials and for further details.
役に立ちましたか？
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Is it safe in terms of health and product safety to store a leftover DMSO ampule covered with parafilm in a flammable cabinet?
1 回答-
DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots and store them under inert gas, in a compatible, tightly sealed container, at a cool storage area, such as 4°C.
DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Product Information Sheet and the Safety Data Sheet at the link below for compatibility of DMSO with various materials and for further details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265
役に立ちましたか？
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Is this anhydrous?
1 回答-
This product is not considered anhydrous. The maximum allowable water content is 0.1% which is reported on the lot-specific Certificate of Analysis. Please see the link below to review the anhydrous DMSO products available:
https://www.sigmaaldrich.com/search/dmso-anhydrous?focus=products&page=1&perpage=30&sort=relevance&term=DMSO%20anhydrous&type=product役に立ちましたか？
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I ordered 10 ml glass vials, used 7 ml, Can I store it in centrifuge tubes? For how long can I use that?
1 回答-
It may be best to store DMSO in amber glass vials for long term storage, as this compound is not compatible with many types of plastics. The compatibility of DMSO with various materials is listed below:
Compatible: LDPE, HDPE, polypropylene, PPCO(polypropylene copolymer), polymethylpentene, nylon, teflon FEP
Moderately compatible: polystyrene, ECTFE/ETFE
Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonateNote that DMSO is hygroscopic and light sensitive. The storage container selected must offer protection from light and moisture uptake. The product is assigned a firm expiration date. Please refer to the lot specific Certificate of Analysis to assume the material is within the expected shelf life.
役に立ちましたか？
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E' solo ad uso per ricerca o utilizzabile anche in diagnostica/human? Grazie
1 回答-
This product is not suitable for diagnostic/human use.
役に立ちましたか？
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Is this product packaged in a bottle with a septa cap?
1 回答-
No, this product is not packaged with a septa cap. It comes in a 125 ml amber glass bottle with a 28 mm solid white cap.
役に立ちましたか？
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Can I use it for PCR?
1 回答-
While this product is tested specifically for use in cell culture, there is no reason to suspect that it would not perform well in PCR applications. The product is NOT tested for amplification, DNAse, or nuclease activity. Should this testing be important, see the link below to product options that include this testing:
D9170 DMSO PCR Reagent:
https://www.sigmaaldrich.com/product/sigma/d9170
D8418 DMSO Molecular Biology Tested
https://www.sigmaaldrich.com/US/en/product/sigma/d8418役に立ちましたか？
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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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