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D5796

Sigma-Aldrich

DMEM - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose

With sodium Bicarbonate and ʟ-glutamine, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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500 ML
$36.00
1 L
$58.90
6 X 500 ML
$188.00
6 X 1 L
$318.00
24 X 500 ML
$677.00

$36.00

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500 ML
$36.00
1 L
$58.90
6 X 500 ML
$188.00
6 X 1 L
$318.00
24 X 500 ML
$677.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

$36.00

In StockDetails

Request a Bulk Order

Product Name

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
D5671D6429D6546
Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

D5796

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

D5671

Dulbecco′s Modified Eagle′s Medium - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

D6429

Dulbecco′s Modified Eagle′s Medium - high glucose

Sigma-Aldrich

D6546

Dulbecco′s Modified Eagle′s Medium - high glucose

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

liquid

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

components

NaHCO3: yes
L-glutamine: yes
glucose: high
phenol red: yes
sodium pyruvate: no
HEPES: no

components

L-glutamine: no
glucose: high
NaHCO3: yes
sodium pyruvate: no
phenol red: yes
HEPES: no

components

HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000408002
0000408002
0000402260
0000402260
0000416297

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Questions

1–3 of 3 Questions  

  1. What are the product numbers for DMEM and EMEM used to grow the 293 and 293T cell lines?

    1 answer

    1. To grow the 293 and 293T cells, the following product recommendations are provided:

      For the 293 cell line (product number 85120602), ECACC recommends using EMEM media (EBSS) with 2mM Glutamine, 1% Non Essential Amino Acids (NEAA), and 10% FCS. ECACC uses the product number M4655 for this purpose.

      For the 293T cell line (product number 12022001), ECACC recommends using DMEM media with 2mM Glutamine and 10% Fetal Bovine Serum (FBS). ECACC uses the product number D5796 media for sub-culturing this cell line.

      Helpful?

  2. What is the conc. of L glutamine

    1 answer

    1. The concentration of L-Glutamine in this product is 0.584 g/L. Please see the link below to review the DMEM Formulation Tables: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/340/109/d5796for.pdf

      Helpful?

  3. pH는 몇으로 조정되어 있나요?

    1 answer

    1. The pH specification of this product is 7.0-7.6. The exact value will be reported in the lot specific Certificate of Analysis. Please see the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D5796/RNBL9771.

      Helpful?

Reviews

Active Filters

  1. Columbia, MO
    • Reviews 2
    • Votes 2
    5 out of 5 stars.

    DMEM

    I always feel confident when using Sigma cell culture media.

    Helpful?

  2. 2 Ratings-Only Reviews

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