UN 1477 5.1 / PGII
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
It is media containing the macro- and micronutrients, and vitamins.
Product M5519 is the complete medium whereas M5524 is just the basal salt medium.
When M5519 is on backorder, you can purchase Murashige and Skoog Basal Salt Mixture (Product No. M5524) and Murashige and Skoog Vitamin Powder (Product No. M7150). You will use 4.3 g/L of M5524 (rather than 4.4 g/L of M5519) and 1 mL/L of M7150. When combined, the salt and organic content is the same as Product No. M5519.
This product does not contain any agar.
Precipitates are known to occur, with time, in plant tissue culture media. The precipitates have been analyzed. They are composed of small pale yellow-white particles. Analysis of precipitates indicated a predominance of iron, phosphate, and zinc. The probable cause of the precipitates is the inevitable oxidation of ferrous ions to ferric ions and the presence of unchelated ferric ions. When the solubility of ferric phosphate is exceeded precipitation occurs. There are no reports of detrimental effects on growth and development in plant tissue culture due to the precipitates.
We would not suggest autoclaving the media, because a precipitate may form. It is advisable to add pre-autoclaved water as it contains less oxygen; this reduces the likelihood of oxidation of ferrous to ferric ions.
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