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M5519

Sigma-Aldrich

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture

All Photos(1)
Synonym(s):
MS0 Medium, MSO Medium, MS Basal Medium
MDL number:
MFCD00240976
NACRES:
NA.72

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

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Murashige and Skoog Basal Medium powder, suitable for plant cell culture

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M5519

Murashige and Skoog Basal Medium

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Murashige and Skoog Basal Medium suitable for plant cell culture, with sucrose and agar

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Murashige and Skoog Basal Medium

Murashige and Skoog Basal Medium powder, suitable for plant cell culture, with Gamborg′s vitamins

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M0404

Murashige and Skoog Basal Medium

form

powder

form

powder

form

powder

form

powder

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Application

Murashige and Skoog medium has been used in plant tissue culture medium for plant transformation study.

Formula variant

With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Pictograms

Flame over circleExclamation mark
GHS03,GHS07

Signal Word

Warning

Hazard Statements

H272 - H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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