Merck
  • H3375
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H3375

Sigma-Aldrich

HEPES

≥99.5% (titration)

Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.25

Nível de qualidade

teor

≥99.5% (titration)

forma

crystalline powder

technique(s)

cell culture | mammalian: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubilidade

water: 500 mg/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicação

HEPES has been used:
  • As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
  • As a component of Hanks′ solution used in the degradation testing of Iron
  • As a component of HEPES medium in the transfection of cell culture with plasmids
  • Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
  • As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

Embalagem

25, 100, 250, 500, 500 g
1 kg in poly bottle
5, 25, 50, 100 kg

Outras notas

Go to Redi-Dri Product Listing RDD002
Easily compare specifications for HEPES products with the HEPES specification table.

Informações legais

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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