Merck
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H4034

Sigma-Aldrich

HEPES

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sinônimo(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
Número CAS:
Peso molecular:
238.30
Beilstein:
883043
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.25

grau

BioPerformance Certified

Nível de qualidade

400

teor

≥99.5% (titration)

technique(s)

cell culture | mammalian: suitable

Impurezas

endotoxin and total aerobic microbial count, tested

useful pH range

6.8-8.2

pKa (25 °C)

7.5

traços de cátion

Fe: ≤5 ppm

absorção

≤0.05 at 260 in H2O at 0.1 M
≤0.05 at 280 in H2O at 0.1 M

application(s)

diagnostic assay manufacturing

atividade externa

DNase, RNase, NICKase, protease, none detected

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descrição geral

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

Aplicação

HEPES has been used:
  • To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines
  • As a component of platelet suspension buffer
  • To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
  • As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
  • For the adjustment and maintenance of pH of biological solutions
  • As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
  • For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
  • As a component of keratinocyte and fibroblast culture medium

Embalagem

25 kg in poly drum
1 kg in poly bottle
100, 500 g in poly bottle

Outras notas

Easily compare specifications for HEPES products with the HEPES specification table.

Código de classe de armazenamento

13 - Non Combustible Solids

WGK

WGK 1

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificado de análise

Insira o número de lote para pesquisar o Certificado de análise (COA).

Certificado de origem

Insira o número de lote para pesquisar o Certificado de origem (COO).

The slice overlay assay: a versatile tool to study the influence of extracellular signals on neuronal development.
Polleux F and Ghosh A
Science's STKE : Signal Transduction Knowledge Environment, 136, pl9-pl9 (2002)
Actin filament elasticity and retrograde flow shape the force-velocity relation of motile cells.
Zimmermann J, et al.
Biophysical Journal, 102(2), 287-295 (2012)
Complex thermorheology of living cells.
Schmidt BUS, et al.
New Journal of Physics, 17(7), 073010-073010 (2015)
Use of a new buffer in the culture of animal cells.
Williamson JD and Cox P
The Journal of General Virology, 2, 309-309 (1968)
Airway epithelial cell PPAR? modulates cigarette smoke-induced chemokine expression and emphysema susceptibility in mice.
Solleti SK et al.
American Journal of Physiology. Lung Cellular and Molecular Physiology, 309, L293-L293 (2015)

Protocolos

Protein Interactions

This protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linkers, formaldehyde has the shortest cross-linking span (~2-3 Å) of any cross-linking reagent, thus making it an ideal tool for detecting specific protein-protein interactions with great confidence.

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

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