MilliporeSigma
All Photos(1)

D9785

SAFC

DMEM/Nutrient Mixture F-12 Ham

With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Synonym(s):
Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture
MDL number:
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO3: no
phenol red: no
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.

Packaging

5, 10, 50 L
1 pkg

Quantity

Formulated to contain 14.8 grams of powder per liter of medium.

Reconstitution

Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service