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P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

for use in purification of Histidine-tagged proteins, DMSO solution

MDL number:
NACRES:
NA.77

Quality Level

biological source

synthetic

form

DMSO solution

storage temp.

−20°C

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Specificity

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Packaging

1 mL in poly bottle
5 mL in glass bottle

Application

Optimized and tested for isolation of histidine-tagged proteins. Chelating agents have been omitted to ensure full compatibility with IMAC applications.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Preparation Note

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

Certificate of Analysis

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Certificate of Origin

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SDS

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