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T6791

Sigma-Aldrich

Trizma® base

BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

description

aminopeptidase substrate

Quality Level

product line

BioXtra

Assay

≥99.9% (titration)

form

crystalline

impurities

≤0.005% Insoluble matter

ign. residue (900 °C)

≤0.01% (as SO4)

loss

≤0.5% loss on drying, 110°C

pH

10.5-12.0 (1 M in H2O)

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

H2O: 1 M, colorless

anion traces

chloride (Cl-): ≤0.005%
sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.020 at 280 in H2O at 1 M
≤0.025 at 260 in H2O at 1 M

SMILES string

NC(CO)(CO)CO

application(s)

diagnostic assay manufacturing

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Trizma® base BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)

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Trizma® base

product line

BioXtra

product line

BioChemika

product line

-

product line

-

assay

≥99.9% (titration)

assay

≥99.0% (T)

assay

≥99.7% (T)

assay

≥99.9% (titration)

form

crystalline

form

crystalline

form

crystalline

form

crystalline powder

impurities

≤0.005% Insoluble matter

impurities

-

impurities

-

impurities

DNase, RNase, protease, none detected, Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested, ≤0.2% water (Karl Fischer)

pH

10.5-12.0 (1 M in H2O)

pH

10.5-12.0(4 m in water, 25 °C)

pH

10.5-11.5 (25 °C, 10% in H2O)

pH

10.0-11.5

mp

167-172 °C (lit.)

mp

167-172 °C (lit.), 168-172 °C

mp

167-172 °C (lit.), 168-172 °C

mp

167-172 °C (lit.)

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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