• Home
  • Search Results
  • Phenolic content, antibacterial and antioxidant activities of Erica herbacea L.

Phenolic content, antibacterial and antioxidant activities of Erica herbacea L.

Acta poloniae pharmaceutica (2014-01-05)
Dragana M Vucić, Miroslav R Petković, Branka B Rodić-Grabovac, Olgica D Stefanović, Sava M Vasić, Ljiljana R Comić
ABSTRACT

Antibacterial and antioxidant activity, total phenolic and flavonoid concentrations of aqueous, ethanol and ethyl acetate extracts from the leaves and flowers of Erica herbacea L. were studied. In vitro antibacterial activity of the extracts was determined by macrodilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined. Testing was performed on 30 clinical isolates, including different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris. The values for MIC were in the range from 2.5 mg/mL to 40 mg/mL. The most sensitive bacterial strains were Proteus vulgaris strains. The aqueous extract from E. herbacea was found the most active. The total phenolic content was determined using Folin-Ciocalteu reagent and ranged between 14.98 and 119.88 mg GA/g. The concentration of flavonoids in extracts was determined using spectrophotometric method with aluminium chloride and obtained results varied from 16.19 to 26.90 mg RU/g. Antioxidant activity was monitored spectrophotometrically using DPPH reagent. The highest capacity to neutralize DPPH radicals was found in the aqueous extract from E. herbacea. The results of the total phenolic content determination of the examined extracts indicate that E. herbacea extracts are a rich source of phenolic compounds and also possess a significant antioxidant activity and moderate antibacterial activity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2,2-Diphenyl-1-picrylhydrazyl
Sigma-Aldrich
Gallic acid, 97.5-102.5% (titration)
Sigma-Aldrich
Phenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology
Sigma-Aldrich
Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%
Sigma-Aldrich
Phenol, for molecular biology
Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)
Sigma-Aldrich
Liquified Phenol, ≥89.0%
Sigma-Aldrich
Aluminum chloride hexahydrate, ReagentPlus®, 99%
Sigma-Aldrich
Phenol, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
Phenol, ≥99%
Supelco
Phenol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Phenol solution, Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology
Sigma-Aldrich
Rutin hydrate, ≥94% (HPLC), powder
Supelco
Gallic acid, certified reference material, TraceCERT®
Sigma-Aldrich
Phenol, contains hypophosphorous as stabilizer, loose crystals, ACS reagent, ≥99.0%
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
Supelco
Phenol solution, certified reference material, 500 μg/mL in methanol
Sigma-Aldrich
Phenol, unstabilized, ReagentPlus®, ≥99%
Chlorogenic acid, primary reference standard
Supelco
Phenol, PESTANAL®, analytical standard
Sigma-Aldrich
Phenol, BioUltra, for molecular biology, TE-saturated, ~73% (T)
Sigma-Aldrich
Aluminum chloride hexahydrate, meets EP, BP, USP testing specifications
Sigma-Aldrich
Phenol, puriss., ≥99.5% (GC), meets analytical specification of Ph. Eur., BP, USP, crystalline (detached)
Sigma-Aldrich
Phenol, ≥96.0% (calc. on dry substance, T)
Supelco
Phenol solution, 5000 μg/mL in methanol, certified reference material
Sigma-Aldrich
1,1-Diphenyl-2-picrylhydrazine, 97%
Sigma-Aldrich
Phenol, unstabilized, purified by redistillation, ≥99%
USP
Phenol, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Phenol, natural, 97%, FG
Chlorogenic acid, European Pharmacopoeia (EP) Reference Standard