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Hormones

Cultured cells require a supply of nutrients for growth and differentiation. Mammalian cell culture media contains a balance of pH, salts, carbohydrates, amino acids, vitamins, fatty acids, lipids, growth factors, hormones, trace elements, and serum. Hormones promote cell growth and can act in a cell-type specific manner. Some cells require hormone supplements to grow under in vitro culture.

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Product Description

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I9278

Insulin solution human, sterile-filtered, BioXtra, suitable for cell culture

I0516

Insulin solution from bovine pancreas, 10 mg/mL insulin in 25 mM HEPES, pH 8.2, BioReagent, sterile-filtered, suitable for cell culture

D4902

Dexamethasone, powder, BioReagent, suitable for cell culture, ≥97%

I6634

Insulin from bovine pancreas, powder, BioReagent, suitable for cell culture

H0888

Hydrocortisone, BioReagent, suitable for cell culture

I2643

Insulin human, recombinant, expressed in yeast (proprietary host)

D2915

Dexamethasone-Water Soluble, suitable for cell culture, BioReagent

E2758

β-Estradiol, BioReagent, powder, suitable for cell culture

M5250

Melatonin, powder, ≥98% (TLC)

T6397

3,3′,5-Triiodo-L-thyronine sodium salt, powder, BioReagent, suitable for cell culture

H0135

Hydrocortisone, γ-irradiated, powder, BioXtra, suitable for cell culture

H6909

Hydrocortisone solution, 50 μM, sterile-filtered, BioXtra, suitable for cell culture

I5500

Insulin from bovine pancreas, ≥25 USP units/mg (HPLC), powder

I1882

Insulin from bovine pancreas, γ-irradiated, BioXtra, suitable for cell culture, potency: ≥20 units/mg (USP units), lyophilized powder

P8783

Progesterone, powder, BioReagent, suitable for cell culture

M4135

α-Melanocyte stimulating hormone, ≥97% (HPLC)

P0409

Prostaglandin E₂, synthetic, powder, BioReagent, suitable for cell culture

T2877

3,3′,5-Triiodo-L-thyronine, ≥95% (HPLC), powder

CG10

Chorionic gonadotropin human, lyophilized powder, vial of ~10,000 IU

H0396

Hydrocortisone-Water Soluble, BioReagent, suitable for cell culture

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Cell Culture Grade Hormone Supplements

The hormone dexamethasone is used in cell culture to study apoptosis, cell signaling pathways, cell differentiation, and gene expression.
Erythropoietin promotes the proliferation, differentiation, and survival of erythroid progenitors.
β-Estradiol hormone is used to study cell differentiation and transformation. β-Estradiol has been used for culturing mammary tumor cells and oocytes.
Glucagon is a peptide hormone that is used as a supplement in some culture and animal model applications, such as measuring glucagon response in mice.
Hydrocortisone can be used in epithelial and endothelial adherent cell culture applications.
Insulin is a peptide hormone that is used as a growth factor in many mammalian cell culture systems.
Progesterone has been used as a growth-promoting hormone in epithelial cell culture.
Prostaglandin E1 and prostaglandin E2 are lipid hormones that are used in some mammalian cell culture applications.
3,3′,5′-Triiodo-L-thyronine (T3) and its precursor thyroxine (T4) hormones are produced by the thyroid gland and are used as supplements in some cell culture applications.

Related Product Categories

Classical Media & Buffers

Cell culture media formulations have been optimized over decades of research in response to diverse cellular physiology. Select media such as DMEM, MEM, RPMI-1640, Ham’s F-10 & F-12 formulations, as well as serum-free solutions such as M199.

Supplements for Cell Culture

Cell culture supplements enable the customization of media with defined characteristics to support specific applications and serum-free culture needs. Our culture-grade supplements include ITS formulations, amino acids, vitamins, antibiotics, and reagents qualified for hybridoma and insect cell culture.

Stem Cells

Our complete range of ES and iPS cell-qualified products includes stem cell lines and media for differentiation and maturation, and provides stem cell researchers with convenient and cost-effective solutions for the reliable culture of pluripotent stem cells.

Primary Cells

Primary cells are derived directly from living tissue and closely replicate the physiology of biological systems. Our collection includes blood, patient tumor xenografts, and hepatocytes essential for drug screening and ADME/Tox studies.

Mammalian Cell Lines

Cell lines from human and other mammalian species are essential for modeling disease and biological systems, and are critical tools for protein, antibody, virus, and vaccine production. We offer authenticated, contaminant-free cell lines, many in partnership with ECACC.

Attachment Factors

Attachment factors mimicking the extracellular matrix (ECM) provide important cues to cultured cells that stimulate physiological growth and behavior. A wide array of biological and synthetic polymers can be selected to enhance cell culture conditions.

Related Applications

Mammalian Cell Culture

Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.

Primary Cell Culture

Primary cells isolated directly from human and animal sources maintain physiological relevance of the tissues from which they are derived, and are used for ADME/Tox screening. Find applications and methods for culturing mammalian primary cells in vitro.

Stem Cell Culture

An overview of different stem cell types, stem cell culture basics and stem cell applications in basic research and clinical research applications, such as tissue regeneration, genetic diseases and cancer.

Cell Culture Media Preparation

Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.

Cell Growth & Maintenance

A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.

Cell-Based Assays

Cellular assays, or cell-based assays, are essential tools for measuring cell health, viability, proliferation, migration, invasion, chemotaxis, apoptosis, angiogenesis, oxidative stress, cell signaling and live cell analysis.

Cell Culture Troubleshooting

Expert tips for correcting common problems with cell culture including bacterial and fungal contamination, debris in culture media, cell detachment or clumping, slow growth, and low viability.

Related Product Literature

Cell Culture Manual