Hormones

Cultured cells require a supply of nutrients for growth and differentiation. Mammalian cell culture media contains a balance of pH, salts, carbohydrates, amino acids, vitamins, fatty acids, lipids, growth factors, hormones, trace elements, and serum. Hormones promote cell growth and can act in a cell-type specific manner. Some cells require hormone supplements to grow under in vitro culture.
Products

Insulin solution human
sterile-filtered, BioXtra, suitable for cell culture

Dexamethasone
powder, BioReagent, suitable for cell culture, ≥97%

Insulin solution from bovine pancreas
10 mg/mL insulin in 25 mM HEPES, pH 8.2, BioReagent, sterile-filtered, suitable for cell culture

Insulin from bovine pancreas
powder, BioReagent, suitable for cell culture

Insulin human
recombinant, expressed in yeast (proprietary host)

Hydrocortisone
BioReagent, suitable for cell culture

Dexamethasone-Water Soluble
BioReagent, suitable for cell culture

Insulin from bovine pancreas
γ-irradiated, BioXtra, suitable for cell culture, potency: ≥20 units/mg (USP units), lyophilized powder

Melatonin
powder, ≥98% (TLC)

Hydrocortisone solution
50 μM, sterile-filtered, BioXtra, suitable for cell culture

3,3′,5-Triiodo-L-thyronine sodium salt
powder, BioReagent, suitable for cell culture

Chorionic gonadotropin human
lyophilized powder, vial of ~10,000 IU

β-Estradiol
BioReagent, powder, suitable for cell culture

α-Melanocyte stimulating hormone
≥97% (HPLC)

Hydrocortisone
γ-irradiated, powder, BioXtra, suitable for cell culture

Follicle Stimulating Hormone from human pituitary
~7,000 IU/mg (powder)

Insulin from bovine pancreas
≥25 USP units/mg (HPLC), powder

Progesterone
powder, BioReagent, suitable for cell culture

Insulin human
recombinant, expressed in yeast, γ-irradiated, suitable for cell culture

3,3′,5-Triiodo-L-thyronine
synthetic (organic), ≥95% (HPLC), Thyroid hormone, powder
Cell Culture Grade Hormone Supplements
- The hormone dexamethasone is used in cell culture to study apoptosis, cell signaling pathways, cell differentiation, and gene expression.
- Erythropoietin promotes the proliferation, differentiation, and survival of erythroid progenitors.
- β-Estradiol hormone is used to study cell differentiation and transformation. β-Estradiol has been used for culturing mammary tumor cells and oocytes.
- Glucagon is a peptide hormone that is used as a supplement in some culture and animal model applications, such as measuring glucagon response in mice.
- Hydrocortisone can be used in epithelial and endothelial adherent cell culture applications.
- Insulin is a peptide hormone that is used as a growth factor in many mammalian cell culture systems.
- Progesterone has been used as a growth-promoting hormone in epithelial cell culture.
- Prostaglandin E1 and prostaglandin E2 are lipid hormones that are used in some mammalian cell culture applications.
- 3,3′,5′-Triiodo-L-thyronine (T3) and its precursor thyroxine (T4) hormones are produced by the thyroid gland and are used as supplements in some cell culture applications.
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