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XNAT

Sigma-Aldrich

REDExtract-N-Amp Tissue PCR Kit

sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included

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Synonym(s):
Tissue direct PCR kit, Tissue direct PCR kit with loading dye
EC Number:
NACRES:
NA.55

usage

sufficient for 10 reactions
10 reactions sufficient for 10 amplifications
10 reactions sufficient for 10 extractions
sufficient for 100 reactions
100 reactions sufficient for 100 amplifications
100 reactions sufficient for 100 extractions
sufficient for 1000 reactions
1000 reactions sufficient for 1000 amplifications
1000 reactions sufficient for 1000 extractions

feature

dNTPs included
hotstart

technique(s)

PCR: suitable

color

red

shipped in

wet ice

storage temp.

−20°C

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XNAT2RXNAPRXNAPS
usage

sufficient for 10 reactions

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

usage

sufficient for 10 amplifications, sufficient for 10 extractions, sufficient for 10 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

color

red

color

colorless

color

red

color

red

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

The REDExtract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva. There is no need for mechanical disruption, organic extraction, column purification, or precipitation of the DNA. The kit includes a PCR ReadyMix, specially formulated for direct PCR amplification from the extract. This formulation uses an antibody-based hot start, for specific amplification. The REDExtract-N-Amp Tissue PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp tissue PCR kit has been used for DNA extraction and in polymerase chain reaction (PCR).

Features and Benefits

  • Perfect for quick genomic DNA isolation for genotyping
  • Single-step extraction of genomic DNA for PCR in 15 minutes
  • PCR ReadyMix, specially formulated for amplification directly from extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • Extract stable at 4oC for at least 6 months
  • REDExtract-N-Amp contains a tracking dye for convenient and direct loading of PCR reactions onto agarose gels for analysis

Components

Kits contain Tissue Preparation Solution, Extraction Solution, and REDExtract-N-Amp PCR Reaction Mix.

Other Notes

The REDExtract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.
For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Principle

The kit comes with validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and REDExtract-N-Amp PCR ReadyMix, included in the kit.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Application

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Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Pascual Torres et al.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 17(1), 269-281 (2019-11-23)
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Molecular phylogenetics and evolution, 75, 219-226 (2014-02-18)
Cyclidiids and thigmotrichids are two diverse groups of scuticociliates, a diverse clade of ciliates that is often difficult to investigate due to the small size and conserved morphology among its members. Compared to other groups (e.g. hypotrichs and oligotrichs), the
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The gut microflora of the honey bee, Apis mellifera, is receiving increasing attention as a potential determinant of the bees' health and their efficacy as pollinators. Studies have focused primarily on the microbial taxa that appear numerically dominant in the
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Articles

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocols

The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.

The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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