P4334
Anti-Protein Kinase Cα antibody produced in rabbit
whole antiserum
Synonym(s):
Anti-PKCα
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.2 mL | Available to ship TODAYfromMILWAUKEE | $532.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, WB
Citations:
135
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
mol wt
antigen 80 kDa
contains
15 mM sodium azide
species reactivity
mouse, rat
technique(s)
microarray: suitable, western blot: 1:20,000 using mouse NIH 3T3 fibroblast lysate, western blot: 1:50,000 using rat brain extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Prkca(18750)
rat ... Prkca(24680)
General description
Anti-Protein Kinase Cα is produced in rabbit using a synthetic peptide conjugated to keyhole limpet hemocyanin (KLH) as the immunogen. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors, and neurotransmitters.
PKC α is a conventional isoform of Protein Kinase C (76-93 kD) and a member of serine/threonine (Ser/Thr) protein kinases. It is expressed ubiquitously in tissues and is the major isoform of PKC in fibroblasts. Stimulation and overexpression of PKC α increases the growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues. It is also useful for detecting PKC α isoenzyme in brain tissue and cell extracts. Furthermore, it can be used in chemiluminescence detection systems to detect PKC α. Rabbit anti-protein kinase c α antibody reacts specifically with PKC α (80 kD) of rat brain extract or NIH3T3 mouse fibroblast lysate.
Immunogen
Synthetic peptide corresponding to amino acids from the C-terminal variable (V5) region of rat PKC α conjugated to KLH.
Application
Anti-Protein Kinase C α has been used in immunoblotting and immunohistochemistry.
Anti-Protein Kinase Cα antibody produced in rabbit has been used in light microscopic—immunocytochemical analysis and immunofluorescent labelling.
Biochem/physiol Actions
Protein Kinase C (PKC) plays a crucial role in signal transduction leading to cellular regulation, cell growth and differentiation, oncogenesis and modulation of neurotransmission. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced as a result from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors and neurotransmitters. PKC is also the major cellular receptor for the tumor promoting phorbol esters. PKC action is mediated by binding to specific receptors for activated C-kinase (RACKs) and through the phosphorylation of several cellular substrates. Overexpression and stimulation of PKC α leads to enhanced growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues.
The antibody reacts in immunoblotting with PKCα in rat brain extract. Staining is specifically inhibited with PKCα immunizing peptide (659-672).
Physical form
Supplied as a liquid containing 15 mM sodium azide as preservative
Preparation Note
For continuous use, store at 2-8 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| antibody form whole antiserum | antibody form whole antiserum | antibody form whole antiserum | antibody form whole antiserum |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| species reactivity mouse, rat | species reactivity rat | species reactivity human, rat, mouse | species reactivity mouse, human, rat |
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Datasheet
Instructions
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Muller B, et al.
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YueMei Zhang et al.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P4334-.2ML | 04061837914133 |