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T5293

Monoclonal Anti-β-Tubulin antibody produced in mouse

clone 2-28-33, ascites fluid

Synonym(s):

Anti-β-Tubulin Antibody

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100 μL

Available to ship TODAYfromMILWAUKEE

$580.00
0.2 mL

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$727.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00145892
Conjugate:
unconjugated
Clone:
2-28-33, monoclonal
Application:
IF, WB
Citations:
153

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2-28-33, monoclonal

contains

15 mM sodium azide

species reactivity

Ciona intestinalis, rat, chicken, Caenorhabditis elegans, Strongylocentrotus purpuratus, bovine, human

enhanced validation

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:1,000 using cultured chicken fibroblasts or cultured human foreskin fibroblasts, western blot: 1:100

isotype

IgG1

UniProt accession no.

Q9H4B7

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
rat ... Tubb2(29212), Tubb2c(296554)

General description

β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins.Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).

Immunogen

sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Application

Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting and fluorescence immunocytochemical staining.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).
The antibody recognizes the two major and one of the minor β‑tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and β-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-β-Tubulin antibody produced in mouse clone 2-28-33, ascites fluid

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species reactivity

Ciona intestinalis, rat, chicken, Caenorhabditis elegans, Strongylocentrotus purpuratus, bovine, human

species reactivity

Xenopus, chicken, human, hamster, canine, mouse, bovine, rat

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

Gene Information

human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
rat ... Tubb2(29212), Tubb2c(296554)

Gene Information

human ... TUBB(203068)
mouse ... Tubb4(22153)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

clone

2-28-33, monoclonal

clone

SAP.4G5, monoclonal

clone

B-5-1-2, monoclonal

clone

DM1A, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000476622
0000476622
0000426882
0000426882
0000426883

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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

Birth of Piglets After Transfer of Embryos Cryopreserved by Cytoskeletal Stabilization and Vitrification
Dobrinsky JR
Biology of Reproduction, 62, 564-570 (2000)
Tilman Jobst-Schwan et al.
American journal of physiology. Renal physiology, 305(5), F734-F744 (2013-07-05)
The inhibitor of apoptosis protein survivin is a bifunctional molecule that regulates cellular division and survival. We have previously shown that survivin protein can be found at high concentrations in the adult kidney, particularly in the proximal tubules. Here, survivin
Hanna S Radomska et al.
The Journal of clinical investigation, 122(8), 2955-2966 (2012-07-17)
Mutations that activate the fms-like tyrosine kinase 3 (FLT3) receptor are among the most prevalent mutations in acute myeloid leukemias. The oncogenic role of FLT3 mutants has been attributed to the abnormal activation of several downstream signaling pathways, such as
View All Related Papers

Global Trade Item Number

SKUGTIN
T5293-.2ML04061838099921
T5293-100UL04061838026927

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