A3429 and A3179 are the same solution but in different package sizes. No dilution is required for these products, but some customers may prefer a slightly weaker acid differentiation solution. The differentiation time may vary due to staining time and the thickness of the tissue sections being stained.
A3429
Differentiation Solution
for the differentiation of regressive hematoxylin stains
Synonym(s):
Acid Alcohol
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 4 L | Please contact Customer Service for Availability | $229.00 |
About This Item
Quality Level
form
solution
shelf life
40 mo.
color
colorless
application(s)
hematology
histology
storage temp.
room temp
General description
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.
Application
Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
Differentiation solution is employed mainly for the differentiation of regressive hematoxylin stains. It has been used in the following studies:
- the prediction of pulmonary metastasis progression in osteosarcoma patients[1]
- the development of multispectral imaging to detect melanin
Biochem/physiol Actions
In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.
Features and Benefits
- Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
- Results are sharper and more rapid compared to other differentiation methods.
- Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.
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This Item | |||
|---|---|---|---|
| color colorless | color colorless | color - | color - |
| form solution | form solution | form - | form - |
| application(s) hematology | application(s) hematology | application(s) environmental | application(s) environmental |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| shelf life 40 mo. | shelf life 40 mo. | shelf life - | shelf life - |
| storage temp. room temp | storage temp. room temp | storage temp. - | storage temp. - |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
target_organs
Eyes,Central nervous system
Storage Class
3 - Flammable liquids
flash_point_f
71.6 °F
flash_point_c
22 °C
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Nuclear staining with alum hematoxylin
B D Llewellyn
Biotechnic & Histochemistry, 84(9), 159-177 (2009)
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
Andres-Manzano, et al.
Methods in Molecular Biology, 1339 (2015)
M Jesús Andrés-Manzano et al.
Methods in molecular biology (Clifton, N.J.), 1339, 85-99 (2015-10-09)
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A3429-4L | 04061833360910 |
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Do Differentiation Solutions A3179 and A3429 need to be diluted before use?
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