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MITOISO2

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Synonym(s):

Mitochondrial Extraction Kit, Mitochondrial Purification Kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
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Quality Level

200

usage

sufficient for 50 applications (2-5 x 107 cells)

technique(s)

ELISA: suitable, western blot: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

Application

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:
  • Ewing′s Sarcoma Family of Tumor (ESFT) cells
  • blood samples of tuberculosis patients
  • human neuroblastoma cells and glioma cells
  • myotubes[1]
  • HepG2 cells

Features and Benefits

  • The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
  • The isolated mitochondria will mostly have intact inner and outer membranes.
  • Can be used to extract mitochondrial proteins for proteome analysis

Other Notes

  • This product is for R&D use only, not for drug, household, or other uses.
  • Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
  • Use ultrapure water for the preparation of reagents.
  • Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

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This Item
MITOISO1CS0760MIT1000
Mitochondria Isolation Kit sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Sigma-Aldrich

MITOISO2

Mitochondria Isolation Kit

Mitochondria Isolation Kit sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

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MITOISO1

Mitochondria Isolation Kit

Isolated Mitochondria Staining Kit 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

Sigma-Aldrich

CS0760

Isolated Mitochondria Staining Kit

Mitochondria/Cytosol Fractionation Kit This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.

Sigma-Aldrich

MIT1000

Mitochondria/Cytosol Fractionation Kit

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

fractionation: suitable

technique(s)

flow cytometry: suitable, protein staining: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

usage

sufficient for 50 applications (2-5 x 107 cells)

usage

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL)

usage

 kit sufficient for 1000 reactions (using 96 multiwell plates),  kit sufficient for 50 reactions (in a 2 mL cuvette)

usage

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

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Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • C2978
    CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL
    SDS
  • P8340
    Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL
    SDS
  • C0356
    Protein Extraction Reagent Type 4 1 bottle
    SDS

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000480623
0000480623
0000354288
0000302541
0000302541

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Global Trade Item Number

SKUGTIN
MITOISO2-1KT04061834066781

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