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Bouin′s solution

Name: Bouin′s solution
Brand: SIGMA

histological fixative

Synonym(s):

Bouin’s fluid

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About This Item

UNSPSC Code:

12171500

NACRES:

NA.47

MDL number:

MFCD00146169

Pricing and availability is not currently available.

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Properties

Quality Level

200

shelf life

Expiry date on the label

composition

acetic acid, 5% , formaldehyde, 9% , picric acid, 0.9%

color

yellow

application(s)

hematology
histology

storage temp.

room temp

Description

General description

Bouin′s solution is a noncoagulant yellow stain fixative that consists of saturated picric acid, formaldehyde, and acetic acid.

Application

Bouin′s solution is an excellent fixative for preserving soft and delicate structures. It is used as a mordant in various trichrome procedures.
Additionally, it has been used for the following purposes-

staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
fixation of rat ovaries for the observing follicle cells using microscopy
fixation of gonads of crab for morphological and histological analysis

Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Biochem/physiol Actions

Bouin′s solution causes shrinkage of tissues when used as a preservative due to its picric acid component. It preserves morphological and nuclear information in tissues upon fixation.

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This Item	32857	HT101128	21819
Sigma-Aldrich HT10132 Bouin′s solution	Sigma-Aldrich 32857 Wright Stain solution	Sigma-Aldrich HT101128 Bouin′s solution	Sigma-Aldrich 21819 Carbol-Fuchsin solution according to Kinyoun
color yellow	color -	color yellow	color red to very dark red
application(s) hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) hematology histology	application(s) diagnostic assay manufacturing hematology histology
shelf life Expiry date on the label	shelf life -	shelf life Expiry date on the label	shelf life -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
composition acetic acid, 5% , picric acid, 0.9% , formaldehyde, 9%	composition -	composition acetic acid, 5% , formaldehyde, 9% , picric acid, 0.9%	composition fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)
storage temp. room temp	storage temp. room temp	storage temp. room temp	storage temp. room temp

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0000525704

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Molecular evidence for distinct modes of nutrient acquisition between visceral and neurotropic schistosomes of birds.

Roman Leontovyč et al.

Scientific reports, 9(1), 1347-1347 (2019-02-06)

Trichobilharzia species are parasitic flatworms (called schistosomes or flukes) that cause important diseases in birds and humans, but very little is known about their molecular biology. Here, using a transcriptomics-bioinformatics-based approach, we explored molecular aspects pertaining to the nutritional requirements

Comparison of cryosurvival and spermatogenesis efficiency of cryopreserved neonatal mouse testicular tissue between three vitrification protocols and controlled-rate freezing.

Cengiz Yildiz et al.

Cryobiology, 84, 4-9 (2018-09-10)

Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation

Characterization of male germ cell markers in canine testis.

Won-Young Lee et al.

Animal reproduction science, 182, 1-8 (2017-05-04)

Spermatogenesis begins at puberty and continues throughout a male's life. This process is initiated and maintained by spermatogonial stem cells in the seminiferous tubules, and these cells produce haploid spermatozoa. Markers of male germ cells have been fully identified in

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Global Trade Item Number

SKU	GTIN
HT10132-1L	04061838261830

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