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A8200

Aminopeptidase from Aeromonas proteolytica

lyophilized powder, 50-150 units/mg protein

Synonym(s):

AAP, Aminopeptidase from Vibrio proteolyticus, bacterial leucyl aminopeptidase

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Pack SizeSKUAvailabilityPrice
100 units
Please contact Customer Service for Availability
$574.00
250 units
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$1,270.00

About This Item

CAS Number:
37288-67-8
EC Number:
3.4.11.10 (BRENDA, IUBMB)
UNSPSC Code:
12352204
EC Number:
232-874-6
NACRES:
NA.54
MDL number:
MFCD00166325
Specific activity:
50-150 units/mg protein

$574.00

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grade

Proteomics Grade

Quality Level

300

form

lyophilized powder

specific activity

50-150 units/mg protein

mol wt

29.5 kDa

composition

Protein, ~40% biuret

solubility

H2O: soluble 0.9-1.1 mg/mL, clear, colorless

foreign activity

endopeptidase, essentially free

storage temp.

−20°C

General description

A zinc-containing enzyme.

Application

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.[1]

Biochem/physiol Actions

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn2+ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.[2]
Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.
Catalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Physical form

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Preparation Note

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Other Notes

One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.

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Aminopeptidase from Aeromonas proteolytica lyophilized powder, 50-150 units/mg protein

Sigma-Aldrich

A8200

Aminopeptidase from Aeromonas proteolytica

Pyroglutamate Aminopeptidase from Pyrococcus furiosus recombinant, expressed in E. coli, ~90% (SDS-PAGE), ≥5.0 units/mg protein

Sigma-Aldrich

P6236

Pyroglutamate Aminopeptidase from Pyrococcus furiosus

Aminopeptidase I from Streptomyces griseus lyophilized powder, ≥200 units/mg protein

Sigma-Aldrich

A9934

Aminopeptidase I from Streptomyces griseus

Protease from Bacillus licheniformis Type VIII, lyophilized powder, 7-15 units/mg solid

Sigma-Aldrich

P5380

Protease from Bacillus licheniformis

grade

Proteomics Grade

grade

-

grade

-

grade

-

specific activity

50-150 units/mg protein

specific activity

≥5.0 units/mg protein

specific activity

≥200 units/mg protein

specific activity

7-15 units/mg solid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

solubility

H2O: soluble 0.9-1.1 mg/mL, clear, colorless

solubility

-

solubility

-

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear, H2O: soluble, colorless

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

29.5 kDa

mol wt

24.072 kDa by amino acid sequence, 28 kDa by SDS-PAGE

mol wt

21 kDa by gel filtration, 33 kDa by SDS-PAGE

mol wt

27 kDa

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000529683
0000529683
0000485852
0000485851
0000485852

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James Kahn et al.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 38(4), 238-241 (2011-05-14)
We have recently designed a biochemistry laboratory experiment for the purpose of providing students an advanced experience with enzyme kinetics and the kinetics of binding. Bestatin, a well-known and commercially available general protease inhibitor, is a slow-binding inhibitor of aminopeptidase
Gudrun Schürer et al.
Biochemistry, 43(18), 5414-5427 (2004-05-05)
The aminopeptidase of Aeromonas proteolytica (AAP) belongs to the group of metallo-hydrolases that require two divalent cations for full activity. Such binuclear metal centers are found in several aminopeptidases, raising the question whether a common mechanism, at least partly, is
G Van Heeke et al.
Biochimica et biophysica acta, 1131(3), 337-340 (1992-07-15)
The gene encoding the Vibrio proteolyticus aminopeptidase was cloned and sequenced and its amino acid sequence was deduced. The gene encodes a 54 kDa protein, larger than the previously reported size of 30 kDa for the purified aminopeptidase. Sequence alignments
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Global Trade Item Number

SKUGTIN
A8200-100UN04061833391303
A8200-250UN04061833391310

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