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Aminopeptidase from Aeromonas proteolytica

Name: Aminopeptidase from <I>Aeromonas proteolytica</I>
Brand: SIGMA
Price: 536 USD
Availability: InStock

lyophilized powder, 50-150 units/mg protein

Synonym(s):

AAP, Aminopeptidase from Vibrio proteolyticus, bacterial leucyl aminopeptidase

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Size/SKU	Availability	Price
100 units	Check Cart for Availability	$536.00
250 units	Check Cart for Availability	$1,070.00

About This Item

CAS Number:

37288-67-8

EC Number:

3.4.11.10 (BRENDA, IUBMB)

UNSPSC Code:

12352204

EC Number:

232-874-6

NACRES:

NA.54

MDL number:

MFCD00166325

Specific activity:

50-150 units/mg protein

$536.00

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Properties

grade

Proteomics Grade

Quality Segment

300

form

lyophilized powder

specific activity

50-150 units/mg protein

mol wt

29.5 kDa

composition

Protein, ~40% biuret

solubility

H₂O: soluble 0.9-1.1 mg/mL, clear, colorless

foreign activity

endopeptidase, essentially free

storage temp.

−20°C

Description

General description

A zinc-containing enzyme.

Application

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.

Biochem/physiol Actions

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn²⁺ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.

Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Catalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Physical form

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Preparation Note

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Other Notes

One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.

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This Item	A4987	164598	MAK613
Sigma-Aldrich A8200 Aminopeptidase from Aeromonas proteolytica	Sigma-Aldrich A4987 Aminopeptidase His-tagged from Vibrio proteolyticus	Sigma-Aldrich 164598 Aminopeptidase M, Porcine Kidney	Sigma-Aldrich MAK613 Leucine Aminopeptidase Activity Assay Kit
description lyophilized powder, 50-150 units/mg protein	description -	description -	description sufficient for 100 colorimetric tests
grade Proteomics Grade	grade Proteomics Grade	grade -	grade -
specific activity 50-150 units/mg protein	specific activity 50-100 U/mg	specific activity -	specific activity -
form lyophilized powder	form powder	form suspension (crystalline)	form -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
solubility H₂O: soluble 0.9-1.1 mg/mL, clear, colorless	solubility -	solubility physiological buffer: soluble, water: soluble	solubility -
mol wt 29.5 kDa	mol wt -	mol wt -	mol wt -

Pictograms

GHS08,GHS07

Signal Word

Danger

Hazard Codes

H315,H319,H334,H335

Precautionary Statements

P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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