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Merck

MP0040A

LookOut® Mycoplasma qPCR Detection Kit

Sinónimos:

Quantiative PCR mycoplasma detection kit

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1 KIT

MXP 18,123.00

MXP 18,123.00


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Acerca de este artículo

UNSPSC Code:
12352207
EC Number:
200-664-3
NACRES:
NA.75

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usage

sufficient for 25 reactions

Quality Level

quality

Not suitable for clinical diagnostic use.

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

PCR: suitable

application(s)

detection
microbiology

storage temp.

2-8°C

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Este artículo
MP0030XNAPSXNAP
technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

usage

sufficient for 25 reactions

usage

 kit sufficient for 5 mL mycoplasma elimination

usage

sufficient for 10 amplifications, sufficient for 10 extractions, sufficient for 10 reactions

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

quality

Not suitable for clinical diagnostic use.

quality

-

quality

-

quality

-

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

-

packaging

-

storage condition

dry at room temperature

storage condition

protect from light

storage condition

-

storage condition

-

mycoplasma detection, qPCR-based assay

mycoplamsa removal agent, MRA,

XNAP, XNAPE, XNAPR, microbialanalysis
microbialanalysis

XNAPE, XNAPR, XNAPS, microbialanalysis
microbialanalysis

General description

The LookOut Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals.

The primer/probe system detects the highly conserved 23S rRNA operon coding region of the mycoplasma genome. The kit is highly specific and does not detect eukaryotic DNA. The detection spectrum includes most mycoplasma species identified as cell culture contaminants (see Table 2 in the technical bulletin). The kit contains the nucleotide dUTP instead of dTTP and is, therefore, suitable for UNG pretreatment.

This kit already contains Taq polymerase in the Mycoplasma Mix.

Application

qPCR Probe Kit for high quality and reliable quantitative detection of mycoplasma DNA in research, industrial application and product testing. Usable for direct testing of cell cultures and biologicals in combination with cell culture enrichment.

Legal Information

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Clase de almacenamiento

10 - Combustible liquids

flash_point_f

188.6 °F

flash_point_c

87 °C

wgk

WGK 3


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Protocolos

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

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SKUGTIN
MP0040A-1KT04061834066842

Questions

  1. Is this product optimized for mycoplasma detection according to the Guideline for Mycoplasma NAT outlined in chapter 2.6.7 of the European Pharmacopeia in terms of specificity, sensitivity/detection limit, and robustness?

    1 answer
    1. Unfortunately, the product MP0040A is not optimized for mycoplasma detection as per the Guideline for Mycoplasma NAT outlined in chapter 2.6.7 of the European Pharmacopeia in terms of specificity, sensitivity/detection limit, and robustness.

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