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492016

NP-40 Alternative

Synonym(s):

NP-40 Alternative - CAS 9016-45-9 - Calbiochem, Nonylphenyl Polyethylene Glycol

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100 mL
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$95.10
500 mL
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$278.00
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$463.00

About This Item

CAS Number:
9016-45-9
UNSPSC Code:
12161902
NACRES:
NA.25
MDL number:
MFCD00132851

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description

RTECS - MD0905000

Quality Level

200

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

color

clear colorless

CMC

50 - 300 μM

solubility

water: 25 mg/mL, aqueous buffer: soluble

density

1.06 g/mL

application(s)

(Isolating membrane proteins)

shipped in

ambient

storage temp.

15-25°C

SMILES string

O(CCOc1ccc(cc1)CCCCCCCCC)CCO

InChI

1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3

InChI key

BLXVTZPGEOGTGG-UHFFFAOYSA-N

General description

A non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis. Similar in usage to NP-40 and TRITON™ X-100 Detergent(Cat. No. 648462).

Application

NP-40 Alternative has been used:
  • as a component of the lysis buffer for solubilizing proteins from transfected HEK293 cells.
  • as a component of the wash buffer for efficient solubilization of biotinylated plasma proteins in plasma protein profiling and chromatin immunoprecipitation (ChIP).
  • for the purification of doublet microtubules from cilia for single particle cryo-electron microscopy (cryo-EM).

Other Notes

Ripoche, P., et al. 1982. Biochim. Biophys. Acta693, 497.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxicity: Irritant (B)

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This Item
49201856741X45
NP-40 Alternative

Millipore

492016

NP-40 Alternative

NP-40 Alternative, PROTEIN GRADE® Detergent, 10% Solution, Sterile-Filtered A non-ionic surfactant useful for the isolation and purification of functional membrane protein complexes. This detergent has been purified to reduce levels of contaminating aldehydes, metals, peroxides, and salts.

Millipore

492018

NP-40 Alternative, PROTEIN GRADE® Detergent, 10% Solution, Sterile-Filtered

IGEPAL® CA-630

Supelco

56741

IGEPAL® CA-630

Triton™ X-45

Sigma-Aldrich

X45

Triton™ X-45

form

liquid

form

solution

form

liquid

form

-

application(s)

(Isolating membrane proteins)

application(s)

(Isolating membrane proteins)

application(s)

-

application(s)

-

storage temp.

15-25°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

CMC

50 - 300 μM

CMC

50 - 300 μM

CMC

0.08 mM (20-25°C)

CMC

-

density

1.06 g/mL

density

-

density

1.06 g/mL at 20 °C

density

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

pictograms

Exclamation markEnvironment
GHS07,GHS09

Storage Class

11 - Combustible Solids

flash_point_f

503.6 °F - Pensky-Martens closed cup - (own results)

flash_point_c

262 °C - Pensky-Martens closed cup - (own results)

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
492016-100ML07790788050832
492016-1000ML04055977273489
492016-500ML04055977273496
492016-4L04061839404465

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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