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BioTracker Rd1 Hypochlorite Live Cell Dye Antibody, Green

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Pack Size	SKU	Availability	Price
1 mg		Check Cart for Availability	$447.00

About This Item

NACRES:

NA.47

UNSPSC Code:

12352207

$447.00

Check Cart for Availability

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assay

≥98% (HPLC)

form

lyophilized

mol wt

calculated mol wt 1080.94

packaging

pkg of 1 mg

manufacturer/tradename

Millipore

storage condition

desiccated, protect from light

technique(s)

cell based assay: suitable

color

off-white

detection method

fluorometric

shipped in

ambient

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This Item	SCT078	SCT043	SCT244
Sigma-Aldrich SCT080 BioTracker Rd1 Hypochlorite Live Cell Dye Antibody, Green	Sigma-Aldrich SCT078 BioTracker Green HOCl-CM2 Live Cell Dye	Sigma-Aldrich SCT043 BioTracker TP-HOCL1 Live Cell Dye	Sigma-Aldrich SCT244 BioTracker Dual-Color Mitochondria-Lysosome Live Cell Dye
technique(s) cell based assay: suitable	technique(s) cell based assay: suitable	technique(s) cell based assay: suitable	technique(s) cell based assay: suitable, cell culture \| stem cell: suitable, flow cytometry: suitable
color off-white	color yellow	color -	color -
form lyophilized	form lyophilized	form -	form -
assay ≥98% (HPLC)	assay ≥98% (HPLC)	assay ≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis)	assay -
shipped in ambient	shipped in ambient	shipped in ambient	shipped in dry ice
storage temp. −20°C	storage temp. −20°C	storage temp. -10 to -25°C	storage temp. −20°C (−15°C to −25°C)

General description

Hypochlorite (HClO/ClO-) is a reactive oxygen species (ROS) produced by the enzyme myeloperoxidase (MPO), which catalyzes the reaction of hydrogen peroxide (H2O2) and chloride ion (Cl-), and plays an important role in the immune response to defend against invading pathogens. Excessive generation of hypochlorite could lead to oxidative stress, which causes inflammation and can lead to a range of diseases, including cardiovascular diseases, atherosclerosis, osteoarthritis, neuron degeneration and cancers[1].

BioTracker Rd1 Hypochlorite Live Cell Dye is a mitochondria-immobilized turn-on green fluorescent probe for the selective detection of hypochlorite (ClO-). The probe contains a benzoyl acetohydrazide linking site for ClO- recognition, a triphenylphosphonium group for mitochondria targeting, and a reactive methoxymaleimide subunit for mitochondrial fixation[1]. The targeting and fixed properties of the probe enable it to accumulate in mitochondria and prevents leakage of the dye upon the decrease of mitochondrial membrane potential (MMP). This probe may be used to visualize exogenous and endogenous ClO- levels in living cells, tissues, and zebrafish[1].

Reference:
1. Li, M. Y., et. al. (2020). Mitochondria-Immobilized Fluorescent Probe for the Detection of Hypochlorite in Living Cells, Tissues, and Zebrafishes. Analytical chemistry, 92(4), 3262-3269.

Application

Purity ≥ 98% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.

Features and Benefits

Mitochondria-immobilized turn-on green fluorescent probe for the selective detection of hypochlorite.

Physical form

Lyophilized. Off-white solid.

Preparation Note

Store at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening

Other Notes

Live cell fluorescent imaging

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Mitochondria-Immobilized Fluorescent Probe for the Detection of Hypochlorite in Living Cells, Tissues, and Zebrafishes.

Meng-Yang Li et al.

Analytical chemistry, 92(4), 3262-3269 (2020-01-21)

A mitochondria targeting and immobilized fluorescent probe (Rd1) using triphenylphosphonium as the targeting group and methoxymaleimide as the fixed site is designed for the detection of ClO-. The methoxymaleimide fixed group can react with nucleophiles, such as the reactive thiol

Global Trade Item Number

SKU	GTIN
SCT080	04061841827986

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