The UPS FASTA file is accessible from the link below:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/protein-mass-spectrometry/ups1-and-ups2-proteomic
UPS2
Proteomics Dynamic Range Standard Set
Protein Mass Spectrometry Calibration Standard
Synonym(s):
Dynamic Range Standards, Proteomics Standard Set
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About This Item
UNSPSC Code:
12161503
EC Number:
232-650-8
NACRES:
NA.24
Form:
ready-to-use solution
Biological source:
human
Pricing and availability is not currently available.
biological source
human
Quality Level
form
ready-to-use solution
quality
Protein Mass Spectrometry Calibration Standard
concentration
10.6 μg/ampule protein
technique(s)
mass spectrometry (MS): suitable
shipped in
wet ice
storage temp.
−20°C
Related Categories
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) mass spectrometry (MS): suitable | technique(s) - | technique(s) mass spectrometry (MS): suitable | technique(s) mass spectrometry (MS): suitable |
| biological source human | biological source - | biological source human | biological source human |
| concentration 10.6 μg/ampule protein | concentration - | concentration - | concentration - |
| form ready-to-use solution | form ready-to-use solution | form lyophilized powder | form lyophilized powder |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
General description
The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.
Application
Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins,[1] embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes. It has also been used as a standard to spike HeLa cells for label-free quantification.
The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[2] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[1]
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[2] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[1]
Kit Components Also Available Separately
Product No.
Description
SDS
- T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg
related product
signalword
Danger
target_organs
Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
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Lee Dicker et al.
Molecular & cellular proteomics : MCP, 9(12), 2704-2718 (2010-09-09)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics provides a wealth of information about proteins present in biological samples. In bottom-up LC-MS/MS-based proteomics, proteins are enzymatically digested into peptides prior to query by LC-MS/MS. Thus, the information directly available from the LC-MS/MS
Jacek R Wiśniewski et al.
Molecular systems biology, 8, 611-611 (2012-09-13)
We report a proteomic analysis of microdissected material from formalin-fixed and paraffin-embedded colorectal cancer, quantifying > 7500 proteins between patient matched normal mucosa, primary carcinoma, and nodal metastases. Expression levels of 1808 proteins changed significantly between normal and cancer tissues
PANDA: A comprehensive and flexible tool for quantitative proteomics data analysis
Chang C, et al.
Bioinformatics, 35, 898-900 (2018)
Rosemary Yu et al.
Nature communications, 11(1), 1881-1881 (2020-04-22)
Cells maintain reserves in their metabolic and translational capacities as a strategy to quickly respond to changing environments. Here we quantify these reserves by stepwise reducing nitrogen availability in yeast steady-state chemostat cultures, imposing severe restrictions on total cellular protein and
Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A, et al.
Molecular and Cellular Proteomics, 13, 1925-1925 (2014)
Articles
High-throughput proteomics advances with improved analysis methods and mass spectrometry.
Related Content
Standardize research with Universal and Dynamic Proteomics Standards, complex and well-characterized reference standards for mass spectrometry.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| UPS2-1SET | 04061837410345 |
| UPS2-5X1SET | 04061837410352 |
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Regarding SKU# UPS2, can you please send me FASTA file of 48 proteins in UPS2 product? thanks you
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