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D0690

DNA Gyrase from Escherichia coli

aqueous glycerol solution

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100 units
Please contact Customer Service for Availability
$580.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
5.99.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00211877
Biological source:
Escherichia coli
Concentration:
≥2 unit/μL

$580.00

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biological source

Escherichia coli

Quality Segment

200

form

aqueous glycerol solution

mol wt

~374 kDa

concentration

≥2 unit/μL

technique(s)

cell based assay: suitable

UniProt accession no.

P0AES4, P0AES6

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Application

DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

Biochem/physiol Actions

DNA gyrase is supplied as an A2B2 holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.
Can be used to supercoil plasmids.

Other Notes

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.
Solution in 50% Glycerol containing Tris buffer, DTT and EDTA.

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description

aqueous glycerol solution

description

buffered aqueous glycerol solution

description

lyophilized powder, 400-2,000 units/mg protein

description

≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Gene Information

Escherichia coli K12 ... polA(948356)

Gene Information

Escherichia coli K12 ... glnA(948370)

Gene Information

Escherichia coli CFT073 ... mutM(1038243)
Escherichia coli K12 ... mutM(946765)

biological source

Escherichia coli

biological source

-

biological source

-

biological source

Escherichia coli

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

nucleic acid detection: suitable

concentration

≥2 unit/μL

concentration

~3,000 units/mL

concentration

-

concentration

-

application(s)

cell analysis

application(s)

-

application(s)

-

application(s)

genomic analysis

form

aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized powder

form

buffered aqueous glycerol solution

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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