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D0690

DNA Gyrase from Escherichia coli

aqueous glycerol solution

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100 units
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$433.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
5.99.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00211877
Biological source:
Escherichia coli
Concentration:
≥2 unit/μL

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biological source

Escherichia coli

Quality Level

200

form

aqueous glycerol solution

mol wt

~374 kDa

concentration

≥2 unit/μL

technique(s)

cell based assay: suitable

UniProt accession no.

P0AES4, P0AES6

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Application

DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

Biochem/physiol Actions

Can be used to supercoil plasmids.
DNA gyrase is supplied as an A2B2 holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA.[1] DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.[2]

Other Notes

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.
Solution in 50% Glycerol containing Tris buffer, DTT and EDTA.

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DNA Gyrase from Escherichia coli aqueous glycerol solution

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Sigma-Aldrich

G1270

L-Glutamine Synthetase from Escherichia coli

biological source

Escherichia coli

biological source

-

biological source

-

biological source

-

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Gene Information

Escherichia coli K12 ... polA(948356)

Gene Information

Escherichia coli K12 ... polA(948356)

Gene Information

Escherichia coli K12 ... glnA(948370)

application(s)

cell analysis

application(s)

-

application(s)

-

application(s)

-

concentration

≥2 unit/μL

concentration

~3,000 units/mL

concentration

5,000-15,000 units/mL

concentration

-

form

aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized powder

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000410798
0000345000
0000410798
0000345000
SLCQ4389

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Related Content

Data Sheet - D0690

Instructions

Magali Toueille et al.
Journal of proteomics, 75(9), 2588-2600 (2012-03-27)
Compared to radiation-sensitive bacteria, the nucleoids of radiation-resistant Deinococcus species show a higher degree of compaction. Such a condensed nucleoid may contribute to the extreme radiation resistance of Deinococcus by limiting dispersion of radiation-induced DNA fragments. Architectural proteins may play
Teresa Lara-Ortíz et al.
Canadian journal of microbiology, 58(2), 195-199 (2012-01-24)
In Escherichia coli cells, an increase in temperature induces immediate DNA relaxation, followed by the fast recovery of DNA supercoiling. DNA gyrase, proteins synthesized during heat stress, and chaperone DnaK have been proposed to participate in this recovery. However, the
S C Kampranis et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(25), 14416-14421 (1996-12-10)
DNA gyrase is unique among topoisomerases in its ability to introduce negative supercoils into closed-circular DNA. We have demonstrated that deletion of the C-terminal DNA-binding domain of the A subunit of gyrase gives rise to an enzyme that cannot supercoil
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Global Trade Item Number

SKUGTIN
D0690-100UN04061833619407

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