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D0690

DNA Gyrase from Escherichia coli

aqueous glycerol solution

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Sobre este item

UNSPSC Code:
12352204
NACRES:
NA.54
Número CE:
5.99.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00211877
Biological source:
Escherichia coli
Concentration:
≥2 unit/μL
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biological source

Escherichia coli

Quality Segment

200

form

aqueous glycerol solution

mol wt

~374 kDa

concentration

≥2 unit/μL

technique(s)

cell based assay: suitable

UniProt accession no.

P0AES4, P0AES6

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Application

DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

Biochem/physiol Actions

Can be used to supercoil plasmids.
DNA gyrase is supplied as an A2B2 holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA.[1] DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.[2]

Other Notes

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.
Solution in 50% Glycerol containing Tris buffer, DTT and EDTA.

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D8276D9380G1270
DNA Gyrase from Escherichia coli aqueous glycerol solution

Sigma-Aldrich

D0690

DNA Gyrase from Escherichia coli

DNA Polymerase I, Klenow Fragment from Escherichia coli buffered aqueous glycerol solution

Sigma-Aldrich

D8276

DNA Polymerase I, Klenow Fragment from Escherichia coli

DNA Polymerase I from Escherichia coli lysogenic for NM 964 buffered aqueous glycerol solution

Sigma-Aldrich

D9380

DNA Polymerase I from Escherichia coli lysogenic for NM 964

L-Glutamine Synthetase from Escherichia coli lyophilized powder, 400-2,000 units/mg protein

Sigma-Aldrich

G1270

L-Glutamine Synthetase from Escherichia coli

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Gene Information

Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Gene Information

Escherichia coli K12 ... polA(948356)

Gene Information

Escherichia coli K12 ... polA(948356)

Gene Information

Escherichia coli K12 ... glnA(948370)

biological source

Escherichia coli

biological source

-

biological source

-

biological source

-

concentration

≥2 unit/μL

concentration

~3,000 units/mL

concentration

5,000-15,000 units/mL

concentration

-

application(s)

cell analysis

application(s)

-

application(s)

-

application(s)

-

form

aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized powder

Classe de armazenamento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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