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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Please contact Customer Service for Availability | PLN 1,740.00 |
About This Item
Storage temp.:
2-8°C
Shipped in:
wet ice
Quality Level
usage
sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)
shipped in
wet ice
storage temp.
2-8°C
General description
The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.
Application
Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.
Biochem/physiol Actions
ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.
Features and Benefits
- May be used in a variety of assay formats
- Suitable for all antibody forms
- Determines all mouse IgG subclasses, IgA, and IgM
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in dry ice |
| usage sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD) | usage - | usage - | usage - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
Kit Components Only
Product No.
Description
- goat antisera to mouse IgA .2 mL
- goat antisera to mouse IgG3 .2 mL
- goat antisera to mouse IgG1 .2 mL
- goat antisera to mouse IgG2a .2 mL
- goat antisera to mouse IgG2b .2 mL
- goat antisera to mouse IgM .2 mL
Storage Class
10 - Combustible liquids
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Protocols
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Szczegółowe protokoły ELISA obejmują pośrednie i wychwytujące techniki ELISA, zalecając produkty do eksperymentów kanapkowych i pośrednich ELISA.
Related Content
Instructions
Masanori Onda et al.
Journal of immunology (Baltimore, Md. : 1950), 177(12), 8822-8834 (2006-12-05)
Recombinant immunotoxins composed of an Ab Fv fragment joined to a truncated portion of Pseudomonas exotoxin A (termed PE38) have been evaluated in clinical trials for the treatment of various human cancers. Immunotoxin therapy is very effective in hairy cell
Preparation and Characterization of a Monoclonal Antibody with High Affinity for Soluble A β Oligomers
Ying,Z et al.
Hybridoma, 28(5) (2009)
Manu Kurian Mathew et al.
The Journal of veterinary medical science, 81(12), 1753-1762 (2019-10-28)
Equine influenza is a leading cause for respiratory illness in equines. Major control measures involve vaccination which requires continuous harmonization owing to antigenic drift. The present study focused on assessing the protective efficacy of an inactivated recombinant equine influenza virus
Global Trade Item Number
| SKU | GTIN |
|---|---|
| ISO2-1KT | 04061833866184 |



