Protein Sample Prep
Sample preparation is critical to prepare and purify proteins for functional or structural studies. Our complete range of reagents and enzymes for cell lysis and protein extraction provide you with an array of options so that you can put together the perfect extraction protocol for your particular cells and protein. We offer a wide vareity of products for protein purification, including agarose resins, magnetic beads, and FLAG® tag technology. Our membrane-based, protein sample concentration technologies, including tangential flow filtration (TFF)-based separation, enable fast, easy concentration, desalting and buffer exchange.
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Amicon® Devices
Centrifugal and pressurized ultrafiltration devices for protein concentration, desalting, buffer exchange, separation, and diafiltration of biological and environmental samples in preparation for downstream applications.
FLAG purification
Expression vectors, affinity resins, and antibodies for expressing, purifying, and detecting FLAG® tagged fusion proteins.
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Protein extraction kits, cell lysis buffers, and reagents for solubilizing proteins from bacteria, yeast, and insect cultures, as well as plant and mammalian cell cultures and tissue samples.
Protease and phosphatase inhibitors and cocktails to prevent proteolysis and protein dephosphorylation, and to preserve the active state of proteins during cell lysis, protein extraction, and sample preparation.
Antibody purification resins and prepacked columns for lab scale, process scale, and industry scale workflow
Dialyzers, centrifugal ultrafiltration devices, and stirred cells for buffer exchange, dialysis, desalting, and diafiltration of 10 µL – 1,200 mL sample volumes.
Centrifugal and pressurized ultrafiltration devices for high recovery protein concentration and concentration of other biological and environmental samples in preparation for downstream applications.
Affinity purification resins and buffers for purifying His-tagged, GST-tagged, FLAG® tagged, S-tag™, Strep-Tag II, and T7-tagged proteins under native or denaturing conditions using batch or column purification techniques.
Streptavidin and avidin resins and prepacked columns for small- to large-scale purification of biotinylated proteins and antibodies.
Fully validated subcellular fractionation and protein enrichment and depletion kits tailored for proteomics research and downstream applications including activity assays, protein microarrays, immunoblotting, ELISA, 2D gel electrophoresis, and mass spectrometry.
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Benzonase® Nuclease
≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail
Protease Inhibitor Cocktail Tablets provided in a glass vial, Tablets provided in a glass vial
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 10 kDa
PhosSTOP™
sufficient for 10 10mL buffer preparations, sufficient for 20 10mL buffer preparations, suitable for tissue processing, suitable for immunoprecipitation (IP)
cOmplete™, EDTA-free Protease Inhibitor Cocktail
Tablets provided in glass vials
Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 30 kDa
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use with mammalian cell and tissue extracts, DMSO solution
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 10 kDa
Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 3 kDa
Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 3 kDa
ANTI-FLAG® M2 antibody, Mouse monoclonal
1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 30 kDa
Amicon® Ultra Centrifugal Filter, 50 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 50 kDa
Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 100 kDa
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 30 kDa
Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 100 kDa
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail
Tablets provided in EASYpacks
RIPA Buffer
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